http://www.mdpi.com/journal/agriculture Latest open access articles published in Agriculture at http://www.mdpi.com/journal/agriculture http://www.mdpi.com/2077-0472/3/2/299 AquaticHealth.net is an open-source aquatic biosecurity intelligence application. By combining automated data collection and human analysis, AquaticHealth.net provides fast and accurate disease outbreak detection and forecasts, accompanied with nuanced explanations. The system has been online and open to the public since 1 January 2010, it has over 200 registered expert users around the world, and it typically publishes about seven daily reports and two weekly disease alerts. We document the major trends in aquatic animal health that the system has detected over these two years, and conclude with some forecasts for the future. Agriculture 2013-05-17 3 2 Article 10.3390/agriculture3020299 299 309 2077-0472 2013-05-17 doi: 10.3390/agriculture3020299 Aidan Lyon Allan Mooney Geoff Grossel http://www.mdpi.com/2077-0472/3/2/285 Wild rocket [Diplotaxis tenuifolia (L.) DC.] belongs to the Brassicaceae family and has its origin in the Mediterranean region. The effect of conventional and integrated cultivation practices on the nutritional properties and benefits of wild rocket [Diplotaxis tenuifolia (L.) DC.] were studied. Bioactive molecules content (vitamin C, quercetin, lutein), antioxidant properties and bioactivity of polyphenolic extracts from the edible part of rocket in Caco-2 cells were determined. Regarding antioxidant properties, FRAP (Ferric Reducing Antioxidant Power) values ranged from 4.44 ± 0.11 mmol/kg fw to 9.92 ± 0.46 mmol/kg fw for conventional rocket and from 4.13 ± 0.17 fw mmol/kg to 11.02 ± 0.45 mmol/kg fw for integrated rocket. The characteristics of wild rocket as a dietary source of antioxidants have been pointed out. Significant differences in the quality of conventional and integrated rocket have been shown, while no influence of agronomic practice on biological activity was reported. A significant accumulation of cells in G1 phase and a consequent reduction in the S and G2 + M phases were observed in Caco-2 cells treated with rocket polyphenol extract. Agriculture 2013-05-06 3 2 Article 10.3390/agriculture3020285 285 298 2077-0472 2013-05-06 doi: 10.3390/agriculture3020285 Alessandra Durazzo Elena Azzini Maria Lazzè Anna Raguzzini Roberto Pizzala Giuseppe Maiani http://www.mdpi.com/2077-0472/3/2/271 There are currently little or no data on the role of endemic disease control in reducing greenhouse gas (GHG) emissions from livestock. In the present study, we have used an Intergovernmental Panel on Climate Change (IPCC)-compliant model to calculate GHG emissions from naturally grazing lambs under four different anthelmintic drug treatment regimes over a 5-year study period. Treatments were either “monthly” (NST), “strategic” (SPT), “targeted” (TST) or based on “clinical signs” (MT). Commercial sheep farming practices were simulated, with lambs reaching a pre-selected target market weight (38 kg) removed from the analysis as they would no longer contribute to the GHG budget of the flock. Results showed there was a significant treatment effect over all years, with lambs in the MT group consistently taking longer to reach market weight, and an extra 10% emission of CO2e per kg of weight gain over the other treatments. There were no significant differences between the other three treatment strategies (NST, SPT and TST) in terms of production efficiency or cumulated GHG emissions over the experimental period. This study has shown that endemic disease control can contribute to a reduction in GHG emissions from animal agriculture and help reduce the carbon footprint of livestock farming. Agriculture 2013-04-24 3 2 Article 10.3390/agriculture3020271 271 284 2077-0472 2013-04-24 doi: 10.3390/agriculture3020271 Fiona Kenyon Jan Dick Ron Smith Drew Coulter David McBean Philip Skuce http://www.mdpi.com/2077-0472/3/2/253 Foreign animal diseases can have a devastating impact on the American economy and agriculture system, while significantly disrupting the food supply chain, and affecting animal health and public health. Continuity of business during an animal disease outbreak aims to mitigate these agriculture-related losses by facilitating normal business operations through the managed movement of non-infected animals and non-contaminated animal products. During a foreign animal disease outbreak, there are competing objectives of trying to control and contain the outbreak while allowing non-infected premises to continue normal business operations to the greatest extent possible. Using a logic model approach, this article discusses the importance of continuity of business planning during an animal disease outbreak, providing a detailed and transparent theoretical framework for continuity of business planning for animal agriculture stakeholders. The logic model provides a basis for continuity of business planning, which is rapidly gaining focus and interest in the animal emergency management community. This unique logic model offers a framework for effective planning and subsequent evaluation of continuity of business plans and processes, by identifying explicit stakeholders, inputs, and activities, alongside the desired outputs and outcomes of such planning. Agriculture 2013-04-23 3 2 Article 10.3390/agriculture3020253 253 270 2077-0472 2013-04-23 doi: 10.3390/agriculture3020253 Kiana Moore Heather Allen http://www.mdpi.com/2077-0472/3/2/236 A standardized whole cranberry extract (WCE) was used to stabilize a model sunflower-casein emulsion prototype for future formulation activities with a fresh cream cheese product. The WCE contained total organic acids (20% w/w) and polyphenols (5%), the latter consisting of total anthocyanins (10%, w/w) and proanthocyanidins (12% w/w). Antioxidant capacity of the WCE was determined by ORAC, (hydrophilic ORAC = 348.31 ± 33.45 µmol of Trolox equivalents/g; lipophilic ORAC = 11.02 ± 0.85 µmol of Trolox equivalents/g). WCE was effective at stabilizing the model emulsion at a level of 0.375% (w/w), yielding a final pH of 5.6. Generation of initial lipid peroxidation products, hexanal and pentanal was inhibited by 92.4% ± 3.9% and 66.6% ± 5.3% (n = 3), respectively, when emulsions containing WCE were incubated at 50 °C for 90 h. This information was useful for formulating a fresh cream cheese product containing WCE to produce value-added potential and good self-life. The standardized WCE gave a final pH of 5.6 for the cheese premix and also significantly (P < 0.05) lowered both the PV and CD after 28 and 21 days at 4 °C storage, respectively, compared to untreated control. We conclude that there are important functional role(s) for cranberry constituents when presented as a standardized ingredient for producing value-added, stable fresh dairy products. Agriculture 2013-04-10 3 2 Article 10.3390/agriculture3020236 236 252 2077-0472 2013-04-10 doi: 10.3390/agriculture3020236 David Kitts Stephen Tomiuk http://www.mdpi.com/2077-0472/3/2/221 Ticks are well-known parasites that affect livestock productivity. This paper reviews the current knowledge regarding the spread of ticks with their impact in animal health and the limitations to achieve effective control measures. The forecasted trends in climate play an obvious role in promoting the spread of ticks in several regions. It appears that climate warming is pivotal in the spread and colonization of new territories by Rhipicephalus microplus in several regions of Africa. The reported increase in altitude of this tick species in the mountainous regions of Central and South America appears to be driven by such general trends in climate change. This factor, however, is not the only single contributor to the spread of ticks. The poor management of farms, uncontrolled movements of domestic animals, abundance of wild animals, and absence of an adequate framework to capture the ecological plasticity of certain ticks may explain the complexity of the control measures. In this paper, we review several details regarding the relationships of ticks with the environment, wild fauna and competition with other species of ticks. Our intention is to highlight these relationships with the aim to produce a coherent framework to explore tick ecology and its relationship with animal production systems. Agriculture 2013-04-10 3 2 Review 10.3390/agriculture3020221 221 235 2077-0472 2013-04-10 doi: 10.3390/agriculture3020221 Agustín Estrada-Peña Mo Salman http://www.mdpi.com/2077-0472/3/2/210 Mechanistic crop growth models are becoming increasingly important in agricultural research and are extensively used in climate change impact assessments. In such studies, statistics of crop yields are usually evaluated without the explicit consideration of sample size requirements. The purpose of this paper was to identify minimum sample sizes for the estimation of average, standard deviation and skewness of maize and winterwheat yields based on simulations carried out under a range of climate and soil conditions. Our results indicate that 15 years of simulated crop yields are sufficient to estimate average crop yields with a relative error of less than 10% at 95% confidence. Regarding standard deviation and skewness, sample size requirements depend on the degree of symmetry of the underlying population’s distribution. For symmetric distributions, samples of 200 and 1500 yield observations are needed to estimate the crop yields’ standard deviation and skewness coefficient, respectively. Higher degrees of asymmetry increase the sample size requirements relative to the estimation of the standard deviation, while at the same time the sample size requirements relative to the skewness coefficient are decreased. Agriculture 2013-04-02 3 2 Technical Note 10.3390/agriculture3020210 210 220 2077-0472 2013-04-02 doi: 10.3390/agriculture3020210 Niklaus Lehmann Robert Finger Tommy Klein Pierluigi Calanca http://www.mdpi.com/2077-0472/3/1/188 Lettuce (Lactuca sativa L.) is extensively grown and is the most widely used food crop for the called “Fourth Range” of vegetables. Lettuce exhibits healthy properties mainly due to the presence of antioxidant compounds (vitamins C and E, carotenoids, polyphenols) alongside significant fibre content and useful amounts of certain minerals. Lettuce can establish a mutualistic association with arbuscular mycorrhizal fungi (AMF). The establishment of the symbiosis involves a continuous cellular and molecular dialogue between both symbionts, which includes the activation of antioxidant, phenylpropanoid or carotenoid metabolic pathways. The presence of AMF colonizing roots of greenhouse-grown lettuces can induce an accumulation of secondary metabolites, vitamins and minerals in leaves that overcome the dilution effect due to the increased size of mycorrhizal plants. Therefore, AMF would allow the intake of minerals and compounds with antioxidant properties to be enhanced without increasing the consumption of lettuce in the diet. In addition, increased quantities of secondary metabolites may help lettuce plants to withstand biotic and abiotic stresses. Our review discusses the influence exerted by several environmental factors and agronomic practices on the ability of AMF for enhancing the levels of vitamins, nutraceuticals and minerals in leaves of green and red-leaf types of lettuces. Agriculture 2013-03-20 3 1 Review 10.3390/agriculture3010188 188 209 2077-0472 2013-03-20 doi: 10.3390/agriculture3010188 Marouane Baslam Idoia Garmendia Nieves Goicoechea http://www.mdpi.com/2077-0472/3/1/170 Citrus contains nutrients and phytochemicals that may be beneficial for health. We collected citrus production and consumption data and estimated the amount of these compounds that are consumed. We then compared the amounts of citrus and citrus-derived compounds used in studies that suggest a health benefit to the amounts typically found in citrus. Data is scarce, but suggests that citrus consumption might improve indices of antioxidant status, and possibly cardiovascular health and insulin sensitivity. Agriculture 2013-03-19 3 1 Review 10.3390/agriculture3010170 170 187 2077-0472 2013-03-19 doi: 10.3390/agriculture3010170 Tami Turner Betty Burri http://www.mdpi.com/2077-0472/3/1/157 Horse gram [Macrotyloma uniflorum (Lam.) Verdc.] seeds containing high concentrations of fatty acids, flavonols and minerals should provide government, public and private organizations with a nutritious and healthy food for use by malnourished and food deprived people worldwide. Seeds from seven horse gram accessions, geographically adapted to Griffin, GA, USA were analyzed for fatty acid, flavonol, and mineral concentrations using gas chromatography, reverse-phase high performance liquid chromatography, and inductively coupled plasma-optical emission spectroscopy, respectively. Significant year effects occurred for stearic, oleic, linoleic, arachidic, gadoleic, and lignoceric acids. Oleic, linoleic, and linolenic acid ranged from 8.9%–16.8%, 40.3%–45.6%, and 11.6%–14.3%, respectively, as percent of total fatty acids measured (total oil ranged from 2.32% to 2.87%). Seed concentrations of myricetin, quercetin, and kaempferol ranged from 0–36 μg/g DW, 0–27 μg/g DW, and 240–316 μg/g DW, respectively and the only year effect was observed for kaempferol among the horse gram accessions. Year effects were found for Fe, K, Mg, Mn, Ni, and S. Mean concentrations of macrominerals (Ca, K, Mg, P, and S) and microminerals (Cu, Fe, Mn, Ni, and Zn) ranged from 1.3–14 mg/g DW, and 1.0–95.0 μg/g DW, respectively. Several correlations were observed among several fatty acids, flavonols, and minerals. The mono-unsaturated fatty acid, oleic acid correlated significantly with linoleic acid (r = −0.64), arachidic acid (r = −0.61), Ca (r = 0.50) and Zn (r = 0.51, all at P < 0.01). The flavonol, myricetin correlated significantly with quercetin (r = 0.92, P < 0.0001), while quercetin correlated with Ca (r = 0.82, P < 0.0001) and kaempferol correlated with Mg (r = 0.61, P < 0.01). Several mineral correlations were found including Fe with K (r = 0.66) and Mg (r = 0.56, both at P < 0.01). These seven horse gram accessions can be used in breeding programs to facilitate the production of superior cultivars with favorable fatty acid profiles, flavonol content, and mineral compositions. Agriculture 2013-03-14 3 1 Article 10.3390/agriculture3010157 157 169 2077-0472 2013-03-14 doi: 10.3390/agriculture3010157 John Morris Ming Wang Michael Grusak Brandon Tonnis http://www.mdpi.com/2077-0472/3/1/147 Groves are of ecological importance, but can reduce the productivity of pastures. They may be used by cattle for nutrition as well as for comfort and shelter. To describe the importance and to estimate the influence of cattle on groves, the behavior of cattle around trees and shrubs was observed on six semi-open pastures in the mountain range of Thuringia and the Southern Black Forest (Germany). The groves were divided into formations, species and structures. The cattle used the groves more for browsing than rubbing. Significantly preferred species calculated by Chesson-Index were dogwood (Cornus sanguinea), black elder (Sambucus nigra), fly honeysuckle (Lonicera xylosteum), plum (Prunus domestica), osier (Salix viminalis), white beam (Sorbus chamaemespilus), and guelder rose (Viburnum opulus). The browsing preference is discussed in relation to nutritional importance and as self-medication. Cattle suppressed some species according to the utilization frequency, but for other species, there was no correlation. The animals preferred the tree hedges in comparison to the other formations. Hedges were utilized as shelter in extreme weather. In addition, under high browsing pressure, hedges were sustained and regenerated. Hedges on pastures turned out to be important for cattle under several aspects and accordingly should be preserved. Agriculture 2013-03-13 3 1 Article 10.3390/agriculture3010147 147 156 2077-0472 2013-03-13 doi: 10.3390/agriculture3010147 Almut Popp Klaus-Manfred Scheibe http://www.mdpi.com/2077-0472/3/1/131 Potatoes are regarded as a significant antioxidant source in human nutrition. However, different types of environmental stress may affect the level of antioxidants in their tuber tissue. In this study, two purple breeding clones and the yellow fleshed cultivar (cv.) Agave were grown in the glasshouse under control with drought stress conditions for two consecutive years. After harvest, the tubers were analysed for concentrations of antioxidants measured as ascorbic acid equivalent (ACE) and trolox equivalent (TXE) in fresh tissue and after wounding. In addition, the peroxidase enzyme (POD) activities and total amounts of anthocyanins (Ac) were assayed. Drought stress caused a significant decrease in tuber yield but had no significant effect on Ac, POD, ACE and TXE. Wounding stress significantly induced the POD activity in control and drought stressed tubers of all genotypes. Also the ACE and TXE were notably increased by wounding in cv. Agave. This was less pronounced in the purple clones which in general displayed a higher level of antioxidants. The results revealed significant differences between genotypes and that the effect of drought stress on the level of antioxidants is smaller than that of wounding stress. Agriculture 2013-02-28 3 1 Article 10.3390/agriculture3010131 131 146 2077-0472 2013-02-28 doi: 10.3390/agriculture3010131 Christina Wegener Gisela Jansen http://www.mdpi.com/2077-0472/3/1/112 There has been an unprecedented demand for inexpensive plant-derived protein hydrolysates in recent years, owing to their potential nutritional applications. This review examines existing evidence regarding protein hydrolysates from agricultural crops such as wheat, soy, rapeseed, sunflower and barley. The bioactivity of these protein hydrolysates, including antioxidant and anti-inflammatory capabilities are discussed. In addition to evidence regarding their potential to enhance human nutrition, the effect of the hydrolysates on the techno-functional properties of foods will be reviewed. Agriculture 2013-02-25 3 1 Article 10.3390/agriculture3010112 112 130 2077-0472 2013-02-25 doi: 10.3390/agriculture3010112 Aoife McCarthy Yvonne O'Callaghan Nora O'Brien http://www.mdpi.com/2077-0472/3/1/90 Most foods are considered functional in terms of providing nutrients and energy to sustain daily life, but dietary systems that are capable of preventing or remediating a stressed or diseased state are classified as functional foods. Dry beans (Phaseolus vulgaris L.) contain high levels of chemically diverse components (phenols, resistance starch, vitamins, fructooligosaccharides) that have shown to protect against such conditions as oxidative stress, cardiovascular disease, diabetes, metabolic syndrome, and many types of cancer, thereby positioning this legume as an excellent functional food. Moreover, the United States has a rich dry bean history and is currently a top producer of dry beans in the world with pinto beans accounting for the vast majority. Despite these attributes, dry bean consumption in the US remains relatively low. Therefore, the objective of this manuscript is to review dry beans as an important US agricultural crop and as functional food for the present age with an emphasis on pinto beans. Agriculture 2013-02-22 3 1 Review 10.3390/agriculture3010090 90 111 2077-0472 2013-02-22 doi: 10.3390/agriculture3010090 Cristiane Câmara Carlos Urrea Vicki Schlegel http://www.mdpi.com/2077-0472/3/1/72 Corn (Zea mays L.) stover is a potential bioenergy feedstock, but little is known about the impacts of reducing stover return on yield and soil quality in the Northern US Corn Belt. Our study objectives were to measure the impact of three stover return rates (Full (~7.8 Mg ha−1 yr−1), Moderate (~3.8 Mg ha−1 yr−1) or Low (~1.5 Mg ha yr−1) Return) on corn and soybean (Glycine max. L [Merr.]) yields and on soil dynamic properties on a chisel-tilled (Chisel) field, and well- (NT1995) or newly- (NT2005) established no-till managed fields. Stover return rate did not affect corn and soybean yields except under NT1995 where Low Return (2.88 Mg ha−1) reduced yields compared with Full and Moderate Return (3.13 Mg ha−1). In NT1995 at 0–5 cm depth, particulate organic matter in Full Return and Moderate Return (14.3 g kg−1) exceeded Low Return (11.3 g kg−1). In NT2005, acid phosphatase activity was reduced about 20% in Low Return compared to Full Return. Also the Low Return had an increase in erodible-sized dry aggregates at the soil surface compared to Full Return. Three or fewer cycles of stover treatments revealed little evidence for short-term impacts on crop yield, but detected subtle soil changes that indicate repeated harvests may have negative consequences if stover removed. Agriculture 2013-02-06 3 1 Article 10.3390/agriculture3010072 72 89 2077-0472 2013-02-06 doi: 10.3390/agriculture3010072 Jane Johnson Veronica Acosta-Martinez Cynthia Cambardella Nancy Barbour http://www.mdpi.com/2077-0472/3/1/53 The interaction between biofuels and food markets remains a policy issue for a number of reasons. There is a continuing need to understand the role of biofuels in the recent spikes in global food prices. Also, there is an ongoing discussion of changes to biofuel policy as a means to cope with severe weather-induced crop losses. Lastly, there are potential interactions between food markets and advanced biofuels, although most of the latter are expected to be produced from non-food feedstocks. This study reviews the existing literature on the food market impacts of biofuels. Findings suggest that initial conclusions attributing most of the spike in global food prices between 2005 and 2008 to biofuels have been revised. Instead, a multitude of factors, in addition to biofuels, converged during the period. Quantitative estimates of the impacts of biofuels on food markets vary significantly due to differences in modeling approaches, geographical scope, and assumptions about a number of crucial factors. In addition, many studies do not adequately account for the effects of macroeconomic changes, adverse weather conditions and direct market interventions during the recent food price spikes when evaluating the role of biofuels. Agriculture 2013-02-04 3 1 Review 10.3390/agriculture3010053 53 71 2077-0472 2013-02-04 doi: 10.3390/agriculture3010053 Gbadebo Oladosu Siwa Msangi http://www.mdpi.com/2077-0472/3/1/33 Fruits are one of the most important sources of polyphenols for humans, whether they are consumed fresh or as processed products. To improve the phenolic content of fruits, a novel field of interest is based on results obtained using elicitors, agrochemicals which were primarily designed to improve resistance to plant pathogens. Although elicitors do not kill pathogens, they trigger plant defense mechanisms, one of which is to increase the levels of phenolic compounds. Therefore, their application not only allows us to control plant disease but also to increase the phenolic content of plant foodstuffs. Pre- or post-harvest application of the most commonly used elicitors to several fruits is discussed in this review. Agriculture 2013-01-25 3 1 Review 10.3390/agriculture3010033 33 52 2077-0472 2013-01-25 doi: 10.3390/agriculture3010033 Yolanda Ruiz-García Encarna Gómez-Plaza http://www.mdpi.com/2077-0472/3/1/12 Two hardwood species, namely red oak and yellow-poplar, were separated into their bark, sapwood and heartwood components. The samples were tested for calorific value, specific gravity, proximate analysis, mineral composition, chemical composition, ultimate analysis, and thermo-chemical decomposition behavior. In addition, the thermo-chemical decomposition behaviors of cellulose, xylan, and lignin polymers were also tested. Thermo-chemical decomposition behavior was assessed using a thermo-gravimetric (TGA) system by heating the sample from 50 °C to 700 °C at the heating rates of 10, 30 and 50 °C/min under nitrogen. The activation energy was calculated for various fractional conversion values using the isoconversion method. The results showed that char yields of lignin, cellulose and xylan were 41.43%, 4.45% and 1.89%, respectively, at the end of pyrolysis. Furthermore, cellulose, xylan and lignin decomposed dramatically in the temperature range of 320 °C to 360 °C, 150 °C to 230 °C and 100 °C to 410 °C, respectively, with decomposition peaks occurring at 340 °C, 200 °C and 340 °C, respectively. In addition, the maximum activation energy for cellulose was 381 kJ/mol at 360 °C and for xylan it was 348 kJ/mol at 210 °C. Agriculture 2013-01-14 3 1 Article 10.3390/agriculture3010012 12 32 2077-0472 2013-01-14 doi: 10.3390/agriculture3010012 Wenjia Jin Kaushlendra Singh John Zondlo http://www.mdpi.com/2077-0472/3/1/1 Rising demands for food and uncertainties about climate change call for a paradigm shift in water management with a stronger focus on rainfed agriculture. The objective here was to estimate water productivity of different crops under no-till (NT) and conventional till (CT), in order to identify rotations that improve the water productivity of dryland agriculture. We hypothesized that NT and cereal crops would have a positive effect on overall water productivity. Crop yield and water use data were obtained from a 15 year experiment (1993 to 2008) on an entic Haplustoll in the semiarid Pampa, Argentina, with a rotation of wheat (Triticum aestivum L.), corn (Zea mays L.), sunflower (Helianthus annus), and soybean (Glycine max L. Merr.). The results indicated an improved water productivity of all crops under NT compared with that of CT; however, the response of cereals (corn +1.0 kg ha−1 mm−1, wheat +1.3 kg ha−1 mm−1) was higher than that of sunflower (+0.3 kg ha−1 mm−1) and soybean (+0.5 kg ha−1 mm−1). Crop type had a higher impact on water productivity than did tillage system. In agreement with our hypothesis, cereal crops were more efficient (corn 9.8 and wheat 6.9 kg ha−1 mm−1) compared with soybean 2.4 and sunflower 3.9 kg mm−1, but the economic water productivity of sunflower (0.9 US$ ha−1 mm−1) almost equaled that of wheat (1.1 US$ ha−1mm−1) and corn (1.2 US$ ha−1 mm−1). We concluded that the use of the synergy between NT and water efficient crops could be a promising step towards improving food production in semiarid regions. Agriculture 2013-01-07 3 1 Article 10.3390/agriculture3010001 1 11 2077-0472 2013-01-07 doi: 10.3390/agriculture3010001 Elke Noellemeyer Romina Fernández Alberto Quiroga http://www.mdpi.com/2077-0472/2/4/472 Cynara cardunculus (Cc) is a multipurpose species; beyond its use in southwestern European cuisine, it is also used for the production of solid biofuel, seed oil, biodiesel, paper pulp and cheese, as well as animal feed. In addition, Cc has a long tradition of use in folk medicine as a diuretic and liver protector. The value of this species as a source of bioactive compounds is known; however, pharmacological use would further increase its cultivation. The main goal of the current work was to evaluate the potential of Cc as source of anti-carcinogenic phytochemicals. Different methanolic extracts obtained from wild and cultivated plants were tested for antioxidant activity and effect on breast tumor cell viability. The most effective extract, both as antioxidant and inhibition of tumor cell viability, was tested for effects on angiogenesis and tumor cell migration capacity. All the extracts tested had high antioxidant activity; however, only green leaves and dry head extracts exhibit anti-proliferative activity. Green cultivated leaves (GCL) were the most effective extract both as antioxidant and inhibiting the proliferation of tumor cells; it is equally active inhibiting tumor cell migration and in vivo angiogenesis. GCL extract is an effective inhibitor of several key points in tumor development and thus a promising source of anti-carcinogenic phytochemicals. Agriculture 2012-12-19 2 4 Article 10.3390/agriculture2040472 472 492 2077-0472 2012-12-19 doi: 10.3390/agriculture2040472 Zélia Velez Marco Campinho Ângela Guerra Laura García Patricia Ramos Olinda Guerreiro Laura Felício Fernando Schmitt Maria Duarte http://www.mdpi.com/2077-0472/2/4/452 Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide. Agriculture 2012-12-18 2 4 Review 10.3390/agriculture2040452 452 471 2077-0472 2012-12-18 doi: 10.3390/agriculture2040452 Ana Villares Laura Mateo-Vivaracho Eva Guillamón http://www.mdpi.com/2077-0472/2/4/436 Canada has a huge stock of biomass resources, which provides a basis (and a temptation) for development of a major bio-fuels industry. Both federal and provincial governments have engaged in a wide array of subsidies, mandates, and other measures to stimulate production and consumption of biofuels. As a result, biofuels has become a growth industry in Canada with production of ethanol almost 10 times higher than it was ten years earlier. However, this has come at considerable cost to taxpayers. Increased biofuel production has resulted in minimal reduction in greenhouse gases, short run (but not long run) increases in net farm income (that benefited grain and oilseed producers but hurt livestock producers), large increases in the prices of farm land due to the higher grain and oilseed prices, and minimal impacts on rural economic diversification. Agriculture 2012-12-17 2 4 Article 10.3390/agriculture2040436 436 451 2077-0472 2012-12-17 doi: 10.3390/agriculture2040436 Danny Le Roy Kurt Klein http://www.mdpi.com/2077-0472/2/4/414 In this paper, we draw the key linkages between future biofuels growth on agricultural commodity prices, and highlight some of the key uncertainties over OECD fuel and energy policies, and their implications for global agricultural markets and the world food situation. Our results show some of the implications that biofuels expansion has on crop area expansion in regions where environmental concerns exist over land use change and the possible impacts on the environment. We also point to some promising areas for future research and specify some implications for policy interventions. Agriculture 2012-12-11 2 4 Article 10.3390/agriculture2040414 414 435 2077-0472 2012-12-11 doi: 10.3390/agriculture2040414 Simla Tokgoz Wei Zhang Siwa Msangi Prapti Bhandary http://www.mdpi.com/2077-0472/2/4/393 The consumption of tea originated in ancient China over 4000 years ago and is currently the second most popular beverage in the world after water. Tea is an aromatic beverage prepared by pouring hot water over cured leaves of the Camellia sinensis plant. The link between tea intake, most notably green tea, and health has resulted in intense research on the components responsible for preventing the onset of several chronic diseases, including atherosclerosis, cancer, obesity and diabetes. In particular, the high levels of chemically diverse phenols (e.g., phenolic acids, flavonoids) present in tea exhibit potent protective properties against many of these diseases. Although health related research on green tea and its predominant phenol (catechins) has been on-going for decades, major advances have occurred in the last 5–10 years. Therefore, this review focuses on seminal studies reported primarily within the last five years but not extending past ten years on the link between health and green tea with an emphasis on the catechins. Agriculture 2012-12-05 2 4 Review 10.3390/agriculture2040393 393 413 2077-0472 2012-12-05 doi: 10.3390/agriculture2040393 Qin-Yin Shi Vicki Schlegel http://www.mdpi.com/2077-0472/2/4/376 Water is crucial for socio-economic development and healthy ecosystems. With the actual population growth and in view of future water scarcity, development calls for improved sectorial allocation of groundwater and surface water for domestic, agricultural and industrial use. Instead of intensifying the pressure on water resources, leading to conflicts among users and excessive pressure on the environment, sewage effluents, after pre-treatment, provide an alternative nutrient-rich water source for agriculture in the vicinity of cities. Water scarcity often occurs in arid and semiarid regions affected by droughts and large climate variability and where the choice of crop to be grown is limited by the environmental factors. Jatropha has been introduced as a potential renewable energy resource since it is claimed to be drought resistant and can be grown on marginal sites. Sewage effluents provide a source for water and nutrients for cultivating jatropha, a combined plant production/effluent treatment system. Nevertheless, use of sewage effluents for irrigation in arid climates carries the risk of salinization. Thus, potential irrigation with sewage effluents needs to consider both the water requirement of the crop and those needed for controlling salinity build-up in the top soil. Using data from a case study in Southern Morocco, irrigation requirements were calculated using CROPWAT 8.0. We present here crop evapotranspiration during the growing period, required irrigation, the resulting nutrient input and the related risk of salinization from the irrigation of jatropha with sewage effluent. Agriculture 2012-12-04 2 4 Article 10.3390/agriculture2040376 376 392 2077-0472 2012-12-04 doi: 10.3390/agriculture2040376 Arisoa Rajaona Nele Sutterer Folkard Asch http://www.mdpi.com/2077-0472/2/4/359 The objective of this study was to investigate the differences in the oxidative stability during storage of fish oil enriched cream cheeses when fish oil was added either as neat oil or pre-emulsified oil with sodium caseinate, whey protein isolate, or a combination of milk proteins and phospholipids as emulsifier. Results showed that the addition of fish oil decreased the oxidative stability of cream cheeses regardless of the addition method, especially when the cheese was stored longer than five weeks. The oxidative stability of fish oil enriched cream cheeses was highest when fish oil was added as neat oil or in a delivery emulsion prepared with a combination of milk proteins and phospholipids. Adding the fish oil in a delivery emulsion prepared with whey protein or caseinate resulted in a less oxidative stable product. It was furthermore shown that the microstructure of the cream cheeses was affected by fish oil addition, and it was suggested that the change in microstructure was partly responsible for the oxidative stability of the cream cheeses. Agriculture 2012-11-12 2 4 Article 10.3390/agriculture2040359 359 375 2077-0472 2012-11-12 doi: 10.3390/agriculture2040359 Anna Frisenfeldt Horn Ditte Green-Petersen Nina Skall Nielsen Ulf Andersen Grethe Hyldig Louise Helene Søgaard Jensen Andy Horsewell Charlotte Jacobsen http://www.mdpi.com/2077-0472/2/4/339 Plant-based transportation fuels were the focus of extended criticism in the press, especially during 2008 when a portion of the blame for a spike in global food prices was associated with growth of the United States’ corn ethanol industry. The critique is based on an unsophisticated portrayal of the ethical issues at stake in the food security implications of biofuel. Three ethical critiques can be leveled at the food vs. fuel debate. First, although market drivers of biofuels indeed skew consumption of agricultural grains, this is not a problem that is unique to biofuels. Second, the critique does not reflect an adequate understanding of the way that rising food prices and changes in agricultural technology affect the food security of the poorest people. Third, although rising food prices could be beneficial to poor farm producers among the world’s poor, it is unlikely that benefits will materialize in the absence of concerted programs to deliberately select biofuel development strategies that are targeted to strengthen food security for poor and small-holding producers. An adequate agricultural ethics for biofuels will require commitment by both private and public sector biofuel developers to ensure that potentially positive attributes of biofuel development are realized. Agriculture 2012-11-06 2 4 Article 10.3390/agriculture2040339 339 358 2077-0472 2012-11-06 doi: 10.3390/agriculture2040339 Paul Thompson http://www.mdpi.com/2077-0472/2/4/325 Over two-thirds of human water withdrawals are estimated to be used for agricultural production, which is expected to increase as demand for renewable liquid fuels from agricultural crops intensifies. Despite the potential implications of bioenergy crop production on water resources, few data are available on water use of perennial bioenergy grass crops. Therefore, the objective of this study was to compare dry matter yield, water use, and water-use efficiency (WUE) of elephantgrass, energycane, and giant reed, grown under field conditions for two growing seasons in North Central Florida. Using scaled sap flow sensor data, water use ranged from about 850 to 1150 mm during the growing season, and was generally greater for giant reed and less for elephantgrass. Despite similar or greater water use by giant reed, dry biomass yields of 35 to 40 Mg ha−1 were significantly greater for energycane and elephantgrass, resulting in greater WUE. Overall, water use by the bioenergy crops was greater than the rainfall received during the study, indicating that irrigation will be needed in the region to achieve optimal yields. Species differ in water use and WUE and species selection can play an important role with regard to potential consequences for water resources. Agriculture 2012-10-19 2 4 Article 10.3390/agriculture2040325 325 338 2077-0472 2012-10-19 doi: 10.3390/agriculture2040325 John E. Erickson Arkorn Soikaew Lynn E. Sollenberger Jerry M. Bennett http://www.mdpi.com/2077-0472/2/4/316 A review of recent literature pertaining to organic and functional food was conducted according its conceptual background. Functional and organic food both belong to fast growing segments of the European food market. Both are food according to the European food regulations, but organic food is further regulated by the European regulation for organic agriculture and food production. This regulation restricts the number of food additives and limits substantial changes in the food. This may cause problems in changing the food based on single constituents or attributes when applying the concept of functional food to organic food production. Claims of the influence of the food positively on health can only be accepted as true when the claims have been tested and then validated by the EU-Commission. Whereas functional food focuses on product comparison based on specific constituents or attributes, organic food as a whole has no placebo for comparison and effects on environment and society are not part of the health claim regulation. Therefore it seems rather difficult to establish the health claims of organic foods. Consumers buy organic food out of an emotional attitude and associate the food with naturalness. In contrast, the decision for buying functional food is related to rationality and consumers associate functional food with a more technological approach. For this reason, the authors conclude that the concept of functional food seems not to support organic food production in Europe. Agriculture 2012-10-17 2 4 Review 10.3390/agriculture2040316 316 324 2077-0472 2012-10-17 doi: 10.3390/agriculture2040316 Johannes Kahl Aneta Załęcka Angelika Ploeger Susanne Bügel Machteld Huber http://www.mdpi.com/2077-0472/2/4/295 Advanced genetic and biotechnology tools will be required to realize the full potential of food and bioenergy crops. Given current regulatory concerns, many transgenic traits might never be deregulated for commercial release without a robust gene confinement strategy in place. The potential for transgene flow from genetically modified (GM) crops is widely known. Pollen-mediated transfer is a major component of gene flow in flowering plants and therefore a potential avenue for the escape of transgenes from GM crops. One approach for preventing and/or mitigating transgene flow is the production of trait linked pollen sterility. To evaluate the feasibility of generating pollen sterility lines for gene confinement and breeding purposes we tested the utility of a promoter (Zm13Pro) from a maize pollen-specific gene (Zm13) for driving expression of the reporter gene GUS and the cytotoxic gene barnase in transgenic rice (Oryza sativa ssp. Japonica cv. Nipponbare) as a monocot proxy for bioenergy grasses. This study demonstrates that the Zm13 promoter can drive pollen-specific expression in stably transformed rice and may be useful for gametophytic transgene confinement and breeding strategies by pollen sterility in food and bioenergy crops. Agriculture 2012-10-16 2 4 Article 10.3390/agriculture2040295 295 315 2077-0472 2012-10-16 doi: 10.3390/agriculture2040295 Joel Hague Stephen Dellaporta Maria Moreno Chip Longo Kimberly Nelson Albert Kausch http://www.mdpi.com/2077-0472/2/4/282 The aim of this study was to assess the effect that the randomised versus even distribution of excreta (dung and urine) may have on modelling nitrogen (N) losses by leaching, volatilisation and denitrification from a grazing system. A range of stock densities (from 200 to 2000 sheep/ha, equivalent to an annual stocking rate of 3 to 33 dry sheep equivalent (DSE)/ha respectively) were simulated to represent an increasing application of N excreta to a grazed 1 hectare area either distributed randomly or uniformly. This study found that the proportion of annual N inputs lost by denitrification were significantly lower and leaching N losses were higher at high stocking densities compared to if excreta was distributed uniformly. The results of this study indicate that N losses from a sheep grazing system could be adequately modelled assuming uniform distribution of excreta at stocking densities up to 1200 sheep/ha (equivalent to an annual stocking rate of 20 DSE/ha). But at higher stock densities, when N loads are high, the spatial distribution of excreta is important and models need to explicitly deal with the distribution of dung and urine N returns. Agriculture 2012-09-25 2 4 Article 10.3390/agriculture2040282 282 294 2077-0472 2012-09-25 doi: 10.3390/agriculture2040282 Matthew J. Bell Brendan R. Cullen Ian R. Johnson Richard J. Eckard http://www.mdpi.com/2077-0472/2/3/272 The paper summarizes key findings of alternative lines of research on the relationship between food and fuel markets, and identifies gaps between two bodies of literature: one that investigates the relationship between food and fuel prices, and another that investigates the impact of the introduction of biofuels on commodity-food prices. The former body of literature suggests that biofuel prices do not affect food-commodity prices, but the latter suggests it does. We try to explain this gap, and then show that although biofuel was an important contributor to the recent food-price inflation of 2001–2008, its effect on food-commodity prices declined after the recession of 2008/09. We also show that the introduction of cross-price elasticity is important when explaining soybean price, but less so when explaining corn prices. Agriculture 2012-09-24 2 3 Article 10.3390/agriculture2030272 272 281 2077-0472 2012-09-24 doi: 10.3390/agriculture2030272 Gal Hochman Scott Kaplan Deepak Rajagopal David Zilberman http://www.mdpi.com/2077-0472/2/3/259 Plants subjected to abiotic stresses synthesize secondary metabolites with potential application in the functional foods, dietary supplements, pharmaceutical, cosmetics and agrochemical markets. This approach can be extended to horticultural crops. This review describes previous reports regarding the effect of different postharvest abiotic stresses on the accumulation of phenolic compounds. Likewise, the physiological basis for the biosynthesis of phenolic compounds as an abiotic stress response is described. The information presented herein would be useful for growers and the fresh produce market which are interested in finding alternative uses for their crops, especially for those not meeting quality standards and thus are considered as waste. Agriculture 2012-09-24 2 3 Review 10.3390/agriculture2030259 259 271 2077-0472 2012-09-24 doi: 10.3390/agriculture2030259 Daniel A. Jacobo-Velázquez Luis Cisneros-Zevallos http://www.mdpi.com/2077-0472/2/3/244 Chokeberries (Aronia melanocarpa) are nowadays believed to exhibit potential cardioprotective and antidiabetic effects principally due to their high content in bioactive phenolic compounds. The stability of the phenolic compounds was studied during different stages of a juice production line and a method for the valorization of pomace was evaluated. Samples were taken from a commercial juice production plant, extracted and analyzed for phenolic constituents and antioxidant potential. Prototypes of functional food ingredients were produced from the pomace by wet milling and micro-milling. Alongside juice processing, the contents of phenolic berry constituents did not vary to a great extent and the overall antioxidant activity increased by about 34%. A high quality juice and a by-product still rich in polyphenols resulted from the process. The phenolic compounds content and the overall antioxidant activity remained stable when milling and micro-milling the pomace. During coarse milling, extractability of total phenolic compounds increased significantly (40% to 50%). Nanosized materials with averaged particle sizes (x50,0) of about 90 nm were obtained by micro-milling. These materials showed significantly enhanced extractability of total phenolic compounds (25%) and total phenolic acid (30%), as well as antioxidant activity (35%), with unchanged contents of total procyanidins and anthocyanins contents. Agriculture 2012-09-20 2 3 Article 10.3390/agriculture2030244 244 258 2077-0472 2012-09-20 doi: 10.3390/agriculture2030244 Esther Mayer-Miebach Marta Adamiuk Diana Behsnilian http://www.mdpi.com/2077-0472/2/3/228 Aromatic plants, also known as herbs and spices, have been used since antiquity as folk medicine and as preservatives in foods. The best known aromatic plants, such as oregano, rosemary, sage, anise, basil, etc., originate from the Mediterranean area. They contain many biologically active compounds, mainly polyphenolics, which have been found to possess antimicrobial, antioxidant, antiparasitic, antiprotozoal, antifungal, and anti-inflammatory properties. Currently, the demand for these plants and their derivatives has increased because they are natural, eco-friendly and generally recognized as safe products. Therefore, aromatic plants and their extracts have the potential to become new generation substances for human and animal nutrition and health. The purpose of this review is to provide an overview of the literature surrounding the in vivo and in vitro use of aromatic plants. Agriculture 2012-09-20 2 3 Review 10.3390/agriculture2030228 228 243 2077-0472 2012-09-20 doi: 10.3390/agriculture2030228 Efterpi Christaki Eleftherios Bonos Ilias Giannenas Panagiota Florou-Paneri http://www.mdpi.com/2077-0472/2/3/211 Yield losses occurring at the field level, whether due to plant diseases or abiotic stresses, reveal reduced stability of the crop yield potential. The paper argues that the stability of crop yield potential is a trait with a clear genetic component, which can be successfully selected for at the single-plant level and incorporated into high-yielding cultivars. Two novel selection equations with prognostic power are presented, capable to objectively phenotype and evaluate individual plants in real field conditions in the absence of the masking effects of interplant competition and soil heterogeneity. The equations predict performance at the crop stand through the key concept of coefficient of homeostasis and are equally useful for early generation selection and for nonstop selection within finished cultivars in order to continuously incorporate the adaptive (genetic or epigenetic) responses of plants. Exploitation of adaptive responses acquires particular importance in view of the climate change effects on crop productivity and the changing biotic or abiotic micro-environments. Cotton is used as a case study to highlight the potential of nonstop selection for increasing crop yield and for the gradual build-up of disease resistance. In addition, the paper envisions and proposes the formation of international networks of researchers focusing on specific diseases as, for example, the cereal root-rot or the cotton Verticillium wilt that will concurrently use the proposed strategy in their respective environments to select for resistant genotypes, while gaining a deeper understanding of the nature of the genetic or epigenetic changes at the phenotypic and genomic levels. Agriculture 2012-09-07 2 3 Concept Paper 10.3390/agriculture2030211 211 227 2077-0472 2012-09-07 doi: 10.3390/agriculture2030211 Dionysia A. Fasoula http://www.mdpi.com/2077-0472/2/3/204 In recent years, obesity is the most common metabolic disease emerging as a global problem especially in developed nations. The discovery of bioactive compounds from natural plant extracts is one possible way to control obesity and prevent or reduce the risks of getting various obesity-related diseases. In this study, we elucidated that Nepetae spica extract significantly reduced the body weight gain induced through feeding a high-fat diet to C57BL/6 mice. The treatment of Nepetae spica extract significantly reduced the adipose tissue weight to 1.5/100 g of body weight in high-fat mice. When their adipose tissue morphology was investigated for histochemical staining, the distribution of cell size in the high-fat diet groups was hypertrophied compared with those from Nepetae spica extract-treated mice. In addition, in Nepetae spica extract-treated mice, a significant reduction of serum triglyceride and T-cholesterol was observed at to 13% and 16%, respectively. These results suggest that Nepetae spica extract could be useful for prevention or treatment of obesity. Agriculture 2012-08-22 2 3 Short Note 10.3390/agriculture2030204 204 210 2077-0472 2012-08-22 doi: 10.3390/agriculture2030204 Changhyun Roh Min-Kyoung Park Hee-June Shin Insub Kim Jin Kyu Kim Uhee Jung http://www.mdpi.com/2077-0472/2/3/182 Taro leaf blight (caused by the Oomycete Phytophthora colocasiae) is a disease of major importance in many regions of the world where taro is grown. Serious outbreaks of taro leaf blight in Samoa in 1993 and in the last few years in Cameroon, Ghana and Nigeria continue to demonstrate the devastating impact of this disease on the livelihoods and food security of small farmers and rural communities dependent on the crop. The spread of the disease to new geographical areas also poses a major threat to neighbouring countries and taro growing regions still free from the disease. Past research, particularly in the Pacific, has demonstrated that management measures such as chemical and cultural control are largely ineffective and that breeding for disease resistance is the most sustainable approach to manage the disease. Recently, the Pacific and South-east Asian regional taro networks have made excellent progress in developing cultivars resistant to taro leaf blight through enhanced utilization of taro genetic resources and close collaboration between farmers and researchers in breeding programs. These programs have secured vital taro genetic resources for future use. This paper provides an overview of the disease, its origin, distribution, biology, epidemiology, management and global impact. The paper will largely focus on breeding strategies to address the disease including challenges, opportunities and constraints. It also discusses how these breeding experiences and outputs can be scaled up to other geographical areas where the disease has been recently introduced or under threat of introduction. Agriculture 2012-07-16 2 3 Review 10.3390/agriculture2030182 182 203 2077-0472 2012-07-16 doi: 10.3390/agriculture2030182 Davinder Singh Grahame Jackson Danny Hunter Robert Fullerton Vincent Lebot Mary Taylor Tolo Iosefa Tom Okpul Joy Tyson http://www.mdpi.com/2077-0472/2/3/165 One potential means to ameliorate consumer concerns over energy security is to increase the domestic production of alternative fuels. However, in the United States, the public’s attitude toward ethanol, one of the most readily available alternative fuels, has been somewhat ambiguous. This study examines consumer attitudes related to energy security and how import levels influence preferences for ethanol blends using an online survey of fuel consumers across the United States. The results suggest that while consumers generally favor both environmental protection and energy security, they are less clear about how to pursue these goals, with no clear majority agreeing with additional drilling or potential effect of corn ethanol production on food prices. The results do suggest that consumers are willing to pay a premium for fuel blends that contain a lower percentage of imported fuel and that the amount of this premium is influenced by both consumer demographics and views on energy security and environmental issues. Agriculture 2012-07-10 2 3 Article 10.3390/agriculture2030165 165 181 2077-0472 2012-07-10 doi: 10.3390/agriculture2030165 Kimberly Jensen Christopher Clark Burton English Dustin Toliver http://www.mdpi.com/2077-0472/2/3/154 Botrytis cinerea is a necrotrophic fungus infecting over 230 plant species worldwide. This highly adaptable pathogen can afflict agricultural products from seed to storage, causing significant economic losses and instability in the food supply. Small protein virulence factors secreted by B. cinerea during infection play an important role in initiation and spread of disease. BcSnod1 was found to be abundantly expressed upon exposure to media containing strawberry extract. From sequence similarity, BcSnod2 was also identified and both were recognized as members of the Ceratoplatanin family of small phytotoxic proteins. Recombinant BcSnod1 was shown to have a phytotoxic effect and play an important role in pathogenicity while the role of BcSnod2 remains less clear. Both bacterial and yeast production systems are reported, though the bacterial protein is less toxic and mostly unfolded relative to that made in yeast. Compared to BcSnod1, recombinant bacterial BcSnod2 shows similar, but delayed phytotoxicity on tomato leaves. Further studies of these critical virulence factors and their inhibition promise to provide new avenues for crop protection. Agriculture 2012-07-10 2 3 Article 10.3390/agriculture2030154 154 164 2077-0472 2012-07-10 doi: 10.3390/agriculture2030154 Subodh Rathi Hana McFeeters Robert L. McFeeters Maria R. Davis http://www.mdpi.com/2077-0472/2/2/139 The effect of phosphorus (P) deficiency on phosphatases activities in N2-fixing legumes has been widely studied in hydroponic culture. However, the response of acid phosphatase (APase) and phytase in rhizosphere, nodules and seeds of Phaseolus vulgaris to low soil’s P-availability is not yet fully understood. In this study, six genotypes of N2-fixing P. vulgaris were grown under contrasting soil P-availabilities; i.e., low (4.3 mg P kg−1) and sufficient (16.7 mg P kg−1) in the Haouz region of Morocco. At flowering and maturity stages, plants were harvested and analyzed for their phosphatases activities, growth and P content. Results show that, low P decreased nodulation, growth, P uptake and N accumulation in all the genotypes, but to a greater extent in the sensitive recombinant inbreed line 147. In addition, while seed P content was slightly reduced under low P soil; a higher P was noticed in the Flamingo and Contender large seeded-beans (6.15 to 7.11 mg g−1). In these latter genotypes, high APase and phytase activities in seeds and nodules were associated with a significant decline in rhizosphere’s available P. APase activity was mainly stimulated in nodules, whereas phytase activity was highly induced in seeds (77%). In conclusion, the variations of APase and phytase activities in nodules and seeds depend on genotype and can greatly influence the internal utilization of P, which might result in low P soil tolerance in N2-fixing legumes. Agriculture 2012-06-13 2 2 Article 10.3390/agriculture2020139 139 153 2077-0472 2012-06-13 doi: 10.3390/agriculture2020139 Adnane Bargaz Mustapha Faghire Neila Abdi Mohamed Farissi Bouaziz Sifi Jean-Jacques Drevon Mohamed Cherkaoui Ikbal Cherki Ghoulam http://www.mdpi.com/2077-0472/2/2/125 The American cranberry (Vaccinium macrocarpon Ait.) contains rich antioxidants and has significant health benefits in fighting a variety of human diseases. In the past ten years, cranberry growers have reported yellow vine syndrome, which is associated with reduced photosynthetic performance, in the cranberry bogs. It has been found that the yellow vine syndrome of cranberry is associated with nutritional imbalance; it might be an issue for cranberry quality and food security as well as the crop production. This review evaluates the present state of knowledge of yellow vine syndrome, together with recent advances that are resulting from an improved mechanistic understanding and a possible solution that will be of considerable value to cranberry growers. This review also includes results from the author’s own laboratory. Water stress, nutritional imbalance, and photoinhibition are the likely reasons for producing yellow vine of cranberry. Future endeavors should be placed on the combination of genetic, biochemical, and biophysical techniques at the molecular level and plant physiology at the field and greenhouse level. This may provide specific information in order to understand the molecular details of yellow vine of cranberry as well as a tool for guiding future breeding efforts and management practices. Agriculture 2012-06-07 2 2 Review 10.3390/agriculture2020125 125 138 2077-0472 2012-06-07 doi: 10.3390/agriculture2020125 Harvey J. M. Hou http://www.mdpi.com/2077-0472/2/2/109 Biological control of fungal plant pathogens can improve global food availability, one of the three pillars of food security, by reducing crop losses, particularly for low-income farmers. However, the interrelationships of many environmental variables can result in multiple interactions among the organisms and their environment, several of which might contribute to effective biological control. Here, we present an advanced survey of the nature and practice of biological control when it is used to control brown rot in stone fruit. Specifically, we describe the population dynamics of Penicillium frequentans and Epicoccum nigrum and their efficacy as biocontrol agents against brown rot disease under field conditions. The size of P. frequentans population after an application of a P. frequentans conidial formulation during the crop season is bigger than that of E. nigrum following the application of an E. nigrum conidial formulation. Moreover, applications of a P. frequentans conidial formulation during the crop season also caused a higher reduction in the number of Monilinia spp. conidia on the fruit surface than that found after applications of an E. nigrum formulation during the growing season. Agriculture 2012-05-21 2 2 Article 10.3390/agriculture2020109 109 124 2077-0472 2012-05-21 doi: 10.3390/agriculture2020109 Antonieta De Cal Inmaculada Larena Belén Guijarro Paloma Melgarejo http://www.mdpi.com/2077-0472/2/2/87 Earthworm biomass and production in savannas are limited by seasonal precipitation and the lack of organic and nutrient resources; I hypothesize that after a long-term protection of savanna from fire and agricultural activities drastic changes in the physical and chemical characteristics of the soil occur with a concomitant increase in earthworm abundance and activities. Similar changes might occur after a long-term fertilization of savannas with manure. This review article considers the earthworm communities and other soil quality indices in Trachypogon savannas of the Orinoco Basin in an organic agricultural forestal savanna (OAFS) amended with compost over forty years in Puerto Ayacucho, Venezuela, and in an Experimental Station long-term protected (PS) from fire and cattle raising from more than four decades in Central Llanos, Venezuela, comparison is made with results from similar savannas. Long-term additions of organic manure or a long protection have induced significant changes in the soil physical and chemical properties of the natural savanna (NS) soils that induce a significant increase in the density and biomass of earthworm populations. On the other hand, the protection of the savanna promotes an improvement in the physical and chemical properties of the soil, which favors an increase in the density and biomass of earthworms in the PS compared with the NS subjected to recurrent burning and grazing. The results emphasize the importance of appropriate organic matter management and the relevance of earthworms in such agroecosystems. Agriculture 2012-04-10 2 2 Review 10.3390/agriculture2020087 87 108 2077-0472 2012-04-10 doi: 10.3390/agriculture2020087 Danilo López-Hernández http://www.mdpi.com/2077-0472/2/1/73 Biofertilizers are becoming increasingly popular in many countries and for many crops, but very few studies on their effect on grain yield have been conducted in rice. Therefore, we evaluated three different biofertilizers (based on Azospirillum, Trichoderma, or unidentified rhizobacteria) in the Philippines during four cropping seasons between 2009 and 2011, using four different fertilizer rates (100% of the recommended rate [RR], 50% RR, 25% RR, and no fertilizer as Control). The experiments were conducted under fully irrigated conditions in a typical lowland rice environment. Significant yield increases due to biofertilizer use were observed in all experimental seasons with the exception of the 2008/09 DS. However, the effect on rice grain yield varied between biofertilizers, seasons, and fertilizer treatments. In relative terms, the seasonal yield increase across fertilizer treatments was between 5% and 18% for the best biofertilizer (Azospirillum-based), but went up to 24% in individual treatments. Absolute grain yield increases due to biofertilizer were usually below 0.5 t·ha−1, corresponding to an estimated additional N uptake of less than 7.5 kg N ha−1. The biofertilizer effect on yield did not significantly interact with the inorganic fertilizer rate used but the best effects on grain yield were achieved at low to medium fertilizer rates. Nevertheless, positive effects of the biofertilizers even occurred at grain yields up to 5 t·ha−1. However, the trends in our results seem to indicate that biofertilizers might be most helpful in rainfed environments with limited inorganic fertilizer input. However, for use in these target environments, biofertilizers need to be evaluated under conditions with abiotic stresses typical of such systems such as drought, soil acidity, or low soil fertility. Agriculture 2012-03-14 2 1 Article 10.3390/agriculture2010073 73 86 2077-0472 2012-03-14 doi: 10.3390/agriculture2010073 Niño Paul Meynard Banayo Pompe C. Sta. Cruz Edna A. Aguilar Rodrigo B. Badayos Stephan M. Haefele http://www.mdpi.com/2077-0472/2/1/52 Some tannins sorb to soil and reduce soluble-N. However, we know little about how they interact with organic amendments in soil. Soil (0–5 cm) from plots, which were amended annually with various carbon substances, was treated with water (control) or solutions containing tannins or related phenolic subunits. Treatments included a proanthocyanidin, catechin, tannic acid, β-1,2,3,4,6-penta-O-galloyl-D-glucose (PGG), gallic acid, and methyl gallate. We applied solutions of each of these materials to soil and measured soluble-C and -N in supernatants after application and following extraction with hot water (16 h, 80 °C). Sorption was low for non-tannin phenolics, methyl gallate, gallic acid, and catechin, and unaffected by amendment. Sorption of tannins, proanthocyanidin, tannic acid, and PGG, was higher and greater in plots amended with biosolids or manure. Extraction of soluble-N was not affected by amendment or by catechin, proanthocyanidin, or methyl gallate, but was reduced with PGG, tannic acid and gallic acid. Soil cation exchange capacity increased following treatment with PGG but decreased with gallic acid, irrespective of amendment. Tannins entering soil may thus influence soil organic matter dynamics and nutrient cycling but their impact may be influenced by the composition of soil organic matter. Agriculture 2012-02-27 2 1 Article 10.3390/agriculture2010052 52 72 2077-0472 2012-02-27 doi: 10.3390/agriculture2010052 Jonathan J. Halvorson Hero T. Gollany Ann C. Kennedy Ann E. Hagerman Javier M. Gonzalez Stewart B. Wuest http://www.mdpi.com/2077-0472/2/1/38 Microbial inoculants are becoming more available as sustainable alternatives to fertilizers and other agrichemicals in broad-acre cropping. However, with the exception of legume inoculants little is understood about effective delivery and survival of the inoculum. Legume inoculants are applied to both seed and soil but seed inoculation is the most economical technique. Large quantities of pasture seed in Australia are inoculated by commercial seed coating companies, but the long-term survival of seed-applied inoculum is variable and monitoring of viability requires specialist microbiology skills and facilities. The aim of our research was to define optimum storage conditions for survival of rhizobia on legume seed and evaluate water activity as a means of monitoring shelf-life. The relationship between survival and water activity varied according to seed species, inoculum preparation, coating ingredients, initial water activity and time suggesting that storage conditions would need to be defined for each different combination. Although drying seeds after coating significantly reduced viable numbers of rhizobia, survival of rhizobia on dried commercially coated lucerne seed after 11 weeks was less variable than seeds that had not been dried. The highest numbers were maintained when seeds remained dry with water activities of between 0.47 and 0.38. The quality of inoculated seed could be improved by reducing the death rate of inoculum during preparation and providing optimum storage conditions for long-term survival. Agriculture 2012-02-06 2 1 Article 10.3390/agriculture2010038 38 51 2077-0472 2012-02-06 doi: 10.3390/agriculture2010038 Rosalind Deaker Elizabeth Hartley Greg Gemell http://www.mdpi.com/2077-0472/2/1/25 The knowledge of important parameters for honey characterization is an increasing requirement of consumers and the honey industry. In this respect, differentiation between blossom honeys and some honeydew honeys is still an unresolved task. This study includes the results of physicochemical and melissopalynological analysis of 86 honey samples from north-western Spain. The relationship between the microscopic elements in honey, such as Metschnikowia cells and fungal spores from plant pathogens, together with their physicochemical parameters were analysed. A cluster analysis was performed to differentiate blossom honey samples from honeydew samples. Metschnikowia cells and certain fungal spores were found to be good variables to enable differentiation between blossom honeys and honeydew honeys. Agriculture 2012-01-20 2 1 Article 10.3390/agriculture2010025 25 37 2077-0472 2012-01-20 doi: 10.3390/agriculture2010025 Escuredo Olga Fernández-González María Seijo María Carmen http://www.mdpi.com/2077-0472/2/1/12 With increasing economic and environmental costs associated with fertilizer use, the need for low-input agroecological systems is on the rise. It is well documented that intercropping legumes can increase the supply of nutrients, through N2-fixation and P mobilization. Concurrently, the integration of trees in the agricultural landscape shows increasing evidence of nutrient inputs through efficient biogeochemical cycles. However, little is known about the effects shade has on legume-crop performance. This research aims to determine whether intercropping of the legumes soybean (Glycine max L. Merr.) and alfalfa (Medicago sativa) with wheat (Triticum turgidum durum) is beneficial for performance, particularly under shady conditions associated with tree-based intercropping. Test species were cultivated in hydroponics with a broad nutrient solution and steady state addition of N for 3 weeks. Individual plants were transferred to rhizoboxes with a 2 mm zone of soil for 16 days under (i) full sun or (ii) shade to mimic light levels at the tree-crop interface. Under monocropping, shading was found to significantly decrease wheat biomass. Intercropping wheat with alfalfa under full sun had no negative effect on growth but did increase wheat P uptake as compared to monocropped wheat. In contrast, intercropping wheat with soybean under full sun decreased wheat biomass, suggesting competition. However, under shade, this competitive effect was mitigated, as wheat exhibited similar biomass and higher N and P shoot concentration when associated with soybean as compared to monocropped wheat under lower light levels. This effect may be attributed to reduced biomass of soybean combined with higher soybean N2-fixation under shade. Legume-based intercrops may increase nutrient supply and growth but these beneficial effects will be dependent on matching species selection to light levels under tree-based intercropping. Agriculture 2012-01-19 2 1 Article 10.3390/agriculture2010012 12 24 2077-0472 2012-01-19 doi: 10.3390/agriculture2010012 Meighen Whitehead Marney E. Isaac http://www.mdpi.com/2077-0472/2/1/1 Total sulfur data of various agricultural and food items from the lab of the author, have been compiled to develop an understanding of sulfur levels and ecological cycling in Austria. As sulfur level is not an included factor among the quality criteria of soil and fertilizer composition, the database is rather small. Problems in analytical determinations of total sulfur, in particular digestions, are outlined. As a protein component, sulfur is enriched in matrices of animal origin, in particular in egg white. There is substantial excretion from animals and man via urine. Organic fertilizers (manures, composts) might contribute significantly to the sulfur budget of soils, which is important for organic farming of crops with high sulfur needs. For soils, drainage is a main route of loss of soluble sulfate, thus pot experiments may yield unrealistic sulfur budgets. Agriculture 2012-01-12 2 1 Article 10.3390/agriculture2010001 1 11 2077-0472 2012-01-12 doi: 10.3390/agriculture2010001 Manfred Sager http://www.mdpi.com/2077-0472/1/1/22 This study was aimed at quantifying soybean (Glycine max) nitrogen fixation under reduced tillage (RT) and conventional tillage (CT) in a tropical Ferralsol of the sub-humid zone of western Kenya, using the isotope 15N dilution method. Crop residue (CR) management was a superimposed treatment in soybean-maize rotation and intercropping systems. This study quantified N in abscised soybean leaves. Soybean-N derived from the atmosphere (%NDfA) ranged between 41–65%; it was higher (P < 0.05) in RT (55.6%) than in CT (46.6%). Total fixed-N under ‘RT + CR’ was more than in the other treatments by at least 55% in intercropping and 34% in rotation system. Nitrogen fixed in soybean aboveground parts was 26–48 kg N ha−1 with intercropping and 53–82 kg N ha−1 with rotation. Seasonal litter fall contained about 15 kg N ha−1, with 54% NDfA. Annual nitrogen balances with soybean and maize grain removed were better in RT (−9 to −32 kg N ha−1) than in CT (−40 to −60 kg N ha−1). Application of P increased nodule weight (P < 0.05) by 3 to 16 times over the control. Soybean residues should be returned to the field after harvest to reduce soil N mining. We conclude that ‘RT + CR’ increases biological nitrogen fixation in soybean, over CT, and that phosphorus application is needed for better soybean nodulation in western Kenya. Agriculture 2011-12-20 1 1 Article 10.3390/agriculture1010022 22 37 2077-0472 2011-12-20 doi: 10.3390/agriculture1010022 Job Kihara Christopher Martius Andre Bationo Paul L. G. Vlek http://www.mdpi.com/2077-0472/1/1/4 Significant amounts of manure are produced in the USA; however, information on the changes in ecosystem services related to soil biogeochemical cycling for agroecosystems supported with organic amendments such as manure is limited. A multi-location field study was initiated in Colorado (CO), Kansas (KS) and Kentucky (KY), USA in loam soils to evaluate the effects of manure and tillage practices on enzyme activities that are key to biogeochemical cycling such as β-glucosidase (C cycling), α-galactosidase (C cycling), β-glucosaminidase (C and N cycling) and phosphomonoesterases (P cycling). The treatments were as follows: (i) two years of beef manure applications to a fine sandy loam at different rates (control: 0, low: 34 kg N ha−1 and high: 96 kg N ha−1) and tillage practices in CO; (ii) three years of beef manure applications to a silt loam at different rates (0, low: 67 kg N ha−1 and high: 134 kg N ha−1) and tillage practices in KS and; (iii) three years of poultry and dairy manure applications to a silt loam with different tillage practices at the same rate (403 kg N ha−1) in KY. Tillage practices (none vs. conventional) had no effect on the enzyme activities. Principal Component Analyses (PCA) grouped all enzyme activities with the high beef manure application rate after the first year in CO at 0–5 cm. By the second year, the low and high beef manure rates differed in enzyme activities for the KS soil with no difference between the low rate and control in CO. Since the first year of the KY study, acid phosphatase activity was greater in the poultry treated soil compared to dairy or the control; whereas, C cycling enzyme activities were similar in soil treated with dairy or poultry manure. For all studies, PCAs for soil samples from 5–10 cm depth did not reveal treatment separation until the second year, i.e., only high application rate differed from the other treatments. Results of the study indicated significant responses in C and P cycling enzyme activities to manure applications within two years, suggesting potential benefits to soil biogeochemical cycling essential for the productivity of agroecosystems supported with organic fertilizers. Agriculture 2011-12-09 1 1 Article 10.3390/agriculture1010004 4 21 2077-0472 2011-12-09 doi: 10.3390/agriculture1010004 Veronica Acosta-Martinez Maysoon M. Mikha Karamat R. Sistani Phillip W. Stahlman Joseph G. Benjamin Merle F. Vigil Richie Erickson http://www.mdpi.com/2077-0472/1/1/1 Ensuring food security is an ongoing global challenge. Many forecasts point to a need to increase food production by at least 70% if we are to feed the world’s projected population of nine billion in 2050. Recent volatility in commodity prices and the general upward trend in the cost of food are indicators that global food systems are now driven by demand rather than supply. There are various reasons for this: greater demand for animal protein with economic growth in developing countries; the continuing increase in world population; competition between food and bioenergy crops for land and water; low inventories of world grain stocks; reduced investment in agricultural R&D; and unfavorable weather resulting in a succession of poor harvests around the world. Increasing production of grains, which are the foundation of the human food supply, will have to be achieved through higher crop yields without boosting inputs of land, water and energy. Meeting community expectations for environmental stewardship and sustainability, and adapting food production to increasingly variable climate, add greatly to the challenge. Agriculture 2011-06-08 1 1 Editorial 10.3390/agriculture1010001 1 3 2077-0472 2011-06-08 doi: 10.3390/agriculture1010001 Les Copeland