Next Article in Journal
Structural Origin of the Enhanced Glass-Forming Ability Induced by Microalloying Y in the ZrCuAl Alloy
Next Article in Special Issue
Interaction and Binding Modes of bis-Ruthenium(II) Complex to Synthetic DNAs
Previous Article in Journal
Investigation on Hot Deformation Behavior and Hot Processing Map of BSTMUF601 Super-Alloy
Previous Article in Special Issue
Iron, Aging, and Neurodegeneration
Article Menu

Export Article

Open AccessFeature PaperReview
Metals 2016, 6(3), 71; doi:10.3390/met6030071

Parameters Influencing Zinc in Experimental Systems in Vivo and in Vitro

1
Institute of Immunology, Faculty of Medicine, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany
2
Department of Food Chemistry and Toxicology, Berlin Institute of Technology, Gustav-Meyer-Allee 25, 13355 Berlin, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Grasso Giuseppe
Received: 31 January 2016 / Revised: 7 March 2016 / Accepted: 17 March 2016 / Published: 21 March 2016
(This article belongs to the Special Issue Metallomics)
View Full-Text   |   Download PDF [2390 KB, uploaded 21 March 2016]   |  

Abstract

In recent years, the role of zinc in biological systems has been a subject of intense research. Despite wide increase in our knowledge and understanding of zinc homeostasis, numerous questions remain to be answered, encouraging further research. In particular, the quantification of intracellular zinc ions and fluctuation, as well as the function of zinc in signaling processes are being intensely investigated. The determination of free intracellular zinc ions is difficult and error-prone, as concentrations are extremely low (in the pico- to nanomolar range), but techniques exist involving fluorescent probes and sensors. In spite of zinc deficiency being accepted as a global problem, causing death and disease worldwide, to date there are no markers to reliably assess a person’s zinc status. This review summarizes the difficulties and major pitfalls when working with zinc in in vitro and in vivo research. Additionally, it specifies important aspects for zinc substitution and supplementation, including the bioavailability of zinc and its intestinal absorption. In particular, it is intended to help researchers with yet minor experience working with zinc efficiently set up experiments and avoid commonly occurring mistakes, starting with the choice and preparation of reagents and instrumentation, and concluding with possibilities for measuring the status of zinc in humans. View Full-Text
Keywords: zinc measurement; bioavailability; zinc solubility; zinc probes; artefacts zinc measurement; bioavailability; zinc solubility; zinc probes; artefacts
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ollig, J.; Kloubert, V.; Weßels, I.; Haase, H.; Rink, L. Parameters Influencing Zinc in Experimental Systems in Vivo and in Vitro. Metals 2016, 6, 71.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Metals EISSN 2075-4701 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top