A Blood Meal Enhances Innexin mRNA Expression in the Midgut, Malpighian Tubules, and Ovaries of the Yellow Fever Mosquito Aedes aegypti
Abstract
:1. Introduction
2. Methods
2.1. Mosquito Rearing
2.2. Blood Feeding
2.3. Dissection, RNA Extraction, and cDNA Synthesis
2.4. qPCR
2.5. dsRNA Synthesis and Injections
2.6. Fecundity and Viability Assays
2.7. Data Analysis
3. Results
3.1. mRNA Expression
3.2. RNAi
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Calkins, T.L.; Piermarini, P.M. A Blood Meal Enhances Innexin mRNA Expression in the Midgut, Malpighian Tubules, and Ovaries of the Yellow Fever Mosquito Aedes aegypti. Insects 2017, 8, 122. https://doi.org/10.3390/insects8040122
Calkins TL, Piermarini PM. A Blood Meal Enhances Innexin mRNA Expression in the Midgut, Malpighian Tubules, and Ovaries of the Yellow Fever Mosquito Aedes aegypti. Insects. 2017; 8(4):122. https://doi.org/10.3390/insects8040122
Chicago/Turabian StyleCalkins, Travis L., and Peter M. Piermarini. 2017. "A Blood Meal Enhances Innexin mRNA Expression in the Midgut, Malpighian Tubules, and Ovaries of the Yellow Fever Mosquito Aedes aegypti" Insects 8, no. 4: 122. https://doi.org/10.3390/insects8040122