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Genes 2017, 8(12), 369; doi:10.3390/genes8120369

Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress

1
State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China
2
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, China
*
Authors to whom correspondence should be addressed.
Received: 8 October 2017 / Revised: 23 November 2017 / Accepted: 24 November 2017 / Published: 5 December 2017
(This article belongs to the Special Issue Genetic Regulation of Abiotic Stress Responses)
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Abstract

Small RNAs play an important role in regulating plant responses to abiotic stress. Depending on the method of salt application, whether sudden or gradual, plants may experience either salt shock or salt stress, respectively. In this study, small RNA expression in response to salt shock and long-term salt stress in parallel experiments was described. Cotton small RNA libraries were constructed and sequenced under normal conditions, as well as sudden and gradual salt application. A total of 225 cotton microRNAs (miRNAs) were identified and of these 24 were novel miRNAs. There were 88 and 75 miRNAs with differential expression under the salt shock and long-term salt stress, respectively. Thirty one transcripts were found to be targets of 20 miRNA families. Eight targets showed a negative correlation in expression with their corresponding miRNAs. We also identified two TAS3s with two near-identical 21-nt trans-acting small interfering RNA (tasiRNA)-Auxin Response Factors (ARFs) that coaligned with the phases D7(+) and D8(+) in three Gossypium species. The miR390/tasiRNA-ARFs/ARF4 pathway was identified and showed altered expression under salt stress. The identification of these small RNAs as well as elucidating their functional significance broadens our understanding of post-transcriptional gene regulation in response to salt stress. View Full-Text
Keywords: microRNAs; miR390; retrotransposons; TAS3; ARF4; tasiRNA-ARFs microRNAs; miR390; retrotransposons; TAS3; ARF4; tasiRNA-ARFs
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yin, Z.; Han, X.; Li, Y.; Wang, J.; Wang, D.; Wang, S.; Fu, X.; Ye, W. Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress. Genes 2017, 8, 369.

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