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Genes 2017, 8(12), 341; doi:10.3390/genes8120341

Comparative Analysis of Soybean Root Proteome Reveals Molecular Basis of Differential Carboxylate Efflux under Low Phosphorus Stress

1
Mineral Nutrition Laboratory, Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
2
National Centre for Conservation and Utilization of Blue Green Algae, Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
Present address: ICAR-Sugarcane Breeding Institute, Coimbatore 641007, India.
*
Author to whom correspondence should be addressed.
Received: 9 October 2017 / Revised: 16 November 2017 / Accepted: 20 November 2017 / Published: 30 November 2017
(This article belongs to the Special Issue Genetic Regulation of Abiotic Stress Responses)
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Abstract

Carboxylate efflux from roots is a crucial and differential response of soybean genotypes to low phosphorus (P) stress. Exudation of carboxylic acids including oxalate, citrate, succinate and fumarate was induced under low P stress, particularly in P-efficient soybean genotypes. Enhancement of root length, surface area and volume further improved P acquisition under low P stress. To understand the molecular basis of carboxylate efflux under low P stress, the root proteome of contrasting genotypes (P-efficient: EC-232019 and P-inefficient: EC-113396) was compared. Among a total of 325 spots, 105 (32%) were differentially abundant proteins (DAPs) between sufficient (250 µM) and low P (4 µM) levels. Abundance of 44 (14%) proteins decreased by more than two-fold under low P stress, while 61 (19%) proteins increased by more than two-fold. Protein identification and annotation revealed that the DAPs were involved in a myriad of functions including carboxylic acid synthesis, carbohydrate, protein and lipid metabolism. Proteins with significant abundance included malate dehydrogenase, isocitrate dehydrogenase, phosphoglucomutase, phosphoglycerate mutase, fructokinase, enolase, phosphoglycerate kinase, triosephosphate isomerase, alcohol dehydrogenase, glucan water dikinase, glutamine synthetase and argininosuccinate lyase. Inferences from proteomic analysis suggests the crosstalk between various metabolic pathways implicated in conferring superior P acquisition efficiency under stress. View Full-Text
Keywords: carboxylate efflux; gene expression analysis; Glycine max; mass spectrometry; phosphorus stress; root proteome; two-dimensional gel electrophoresis carboxylate efflux; gene expression analysis; Glycine max; mass spectrometry; phosphorus stress; root proteome; two-dimensional gel electrophoresis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Vengavasi, K.; Pandey, R.; Abraham, G.; Yadav, R.K. Comparative Analysis of Soybean Root Proteome Reveals Molecular Basis of Differential Carboxylate Efflux under Low Phosphorus Stress. Genes 2017, 8, 341.

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