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Genes 2012, 3(4), 779-805; doi:10.3390/genes3040779
Review

The Sound of Silence: RNAi in Poly (ADP-Ribose) Research

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Received: 4 September 2012; in revised form: 5 November 2012 / Accepted: 6 November 2012 / Published: 6 December 2012
(This article belongs to the Special Issue RNA Interference)
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Abstract: Poly(ADP-ribosyl)-ation is a nonprotein posttranslational modification of proteins and plays an integral part in cell physiology and pathology. The metabolism of poly(ADP-ribose) (PAR) is regulated by its synthesis by poly(ADP-ribose) polymerases (PARPs) and on the catabolic side by poly(ADP-ribose) glycohydrolase (PARG). PARPs convert NAD+ molecules into PAR chains that interact covalently or noncovalently with target proteins and thereby modify their structure and functions. PAR synthesis is activated when PARP1 and PARP2 bind to DNA breaks and these two enzymes account for almost all PAR formation after genotoxic stress. PARG cleaves PAR molecules into free PAR and finally ADP-ribose (ADPR) moieties, both acting as messengers in cellular stress signaling. In this review, we discuss the potential of RNAi to manipulate the levels of PARPs and PARG, and consequently those of PAR and ADPR, and compare the results with those obtained after genetic or chemical disruption.
Keywords: ADPR; PAR; PARP1; PARP2; PARG; siRNA ADPR; PAR; PARP1; PARP2; PARG; siRNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Blenn, C.; Wyrsch, P.; Althaus, F.R. The Sound of Silence: RNAi in Poly (ADP-Ribose) Research. Genes 2012, 3, 779-805.

AMA Style

Blenn C, Wyrsch P, Althaus FR. The Sound of Silence: RNAi in Poly (ADP-Ribose) Research. Genes. 2012; 3(4):779-805.

Chicago/Turabian Style

Blenn, Christian; Wyrsch, Philippe; Althaus, Felix R. 2012. "The Sound of Silence: RNAi in Poly (ADP-Ribose) Research." Genes 3, no. 4: 779-805.


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