• Abstract
• Full-Text PDF [645 KB]
• Full-Text XML
• Article Versions Notes

• Article Statistics
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Reiner, O
• Gorelik, A
• Greenman, R
• [+] on Google Scholar
• Reiner, O
• Gorelik, A
• Greenman, R
• [+] on PubMed
• Reiner, O
• Gorelik, A
• Greenman, R

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Genes 2012, 3(4), 759-778; doi:10.3390/genes3040759

Review

Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily

Orly Reiner* , Anna Gorelik and Raanan Greenman

Department of Molecular Genetics, Weizmann Institute of Science, 76100 Rehovot, Israel

* Author to whom correspondence should be addressed.

Received: 27 September 2012; in revised form: 22 October 2012 / Accepted: 31 October 2012 / Published: 21 November 2012

(This article belongs to the Special Issue RNA Interference)

Download PDF Full-Text [645 KB, uploaded 21 November 2012 09:08 CET]

Abstract: The way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the developing brain and compare them with knockout experiments, thus highlighting the advantages and disadvantages using the different systems. Our review will focus on experiments relating to the doublecortin superfamily of proteins.

Keywords: doublecortin; DCX; shRNA; in utero electroporation; neuronal migration

Article Statistics

Cite This Article