Next Article in Journal
Understanding the Molecular Circuitry of Cell Lineage Specification in the Early Mouse Embryo
Next Article in Special Issue
Modeling the Risk of Secondary Malignancies after Radiotherapy
Previous Article in Journal
Special Issue: Gene Conversion in Duplicated Genes
Previous Article in Special Issue
Allelic Imbalances in Radiation—Associated Acute Myeloid Leukemia
Genes 2011, 2(3), 397-419; doi:10.3390/genes2030397
Article

Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer

1
, 1,2
, 1
, 3
, 1,4
, 5
, 1,6
, 1,5,7
, 8
, 1
, 1
 and 1,9,10,*
Received: 23 April 2011; in revised form: 23 May 2011 / Accepted: 16 June 2011 / Published: 28 June 2011
(This article belongs to the Special Issue Radiation-Related Cancer 25 Years After Chernobyl)
View Full-Text   |   Download PDF [770 KB, updated 29 June 2011; original version uploaded 28 June 2011]   |   Browse Figures
Abstract: Recurrent translocations are well known hallmarks of many human solid tumors and hematological disorders, where patient- and breakpoint-specific information may facilitate prognostication and individualized therapy. In thyroid carcinomas, the proto-oncogenes RET and NTRK1 are often found to be activated through chromosomal rearrangements. However, many sporadic tumors and papillary thyroid carcinomas (PTCs) arising in patients with a history of exposure to elevated levels of ionizing irradiation do not carry these known abnormalities. We developed a rapid scheme to screen tumor cell metaphase spreads and identify candidate genes of tumorigenesis and neoplastic progression for subsequent functional studies. Using a series of overnight fluorescence in situ hybridization (FISH) experiments with pools comprised of bacterial artificial chromosome (BAC) clones, it now becomes possible to rapidly refine breakpoint maps and, within one week, progress from the low resolution Spectral Karyotyping (SKY) maps or Giemsa-banding (G-banding) karyotypes to fully integrated, high resolution physical maps including a list of candiate genes in the critical regions.
Keywords: Chernobyl; neoplastic disease; papillary thyroid cancer; translocation; molecular cytogenetics; breakpoint delineation; fluorescence in situ hybridization; bacterial artificial chromosomes Chernobyl; neoplastic disease; papillary thyroid cancer; translocation; molecular cytogenetics; breakpoint delineation; fluorescence in situ hybridization; bacterial artificial chromosomes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Weier, H.-U.; Ito, Y.; Kwan, J.; Smida, J.; Weier, J.F.; Hieber, L.; Lu, C.-M.; Lehmann, L.; Wang, M.; Kassabian, H.J.; Zeng, H.; O’Brien, B. Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer. Genes 2011, 2, 397-419.

AMA Style

Weier H-U, Ito Y, Kwan J, Smida J, Weier JF, Hieber L, Lu C-M, Lehmann L, Wang M, Kassabian HJ, Zeng H, O’Brien B. Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer. Genes. 2011; 2(3):397-419.

Chicago/Turabian Style

Weier, Heinz-Ulrich G.; Ito, Yuko; Kwan, Johnson; Smida, Jan; Weier, Jingly F.; Hieber, Ludwig; Lu, Chun-Mei; Lehmann, Lars; Wang, Mei; Kassabian, Haig J.; Zeng, Hui; O’Brien, Benjamin. 2011. "Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer." Genes 2, no. 3: 397-419.


Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert