Genes 2011, 2(3), 397-419; doi:10.3390/genes2030397

Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer

1email, 1,2email, 1email, 3email, 1,4email, 5email, 1,6email, 1,5,7email, 8email, 1email, 1email and 1,9,10,* email
Received: 23 April 2011; in revised form: 23 May 2011 / Accepted: 16 June 2011 / Published: 28 June 2011
(This article belongs to the Special Issue Radiation-Related Cancer 25 Years After Chernobyl)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Recurrent translocations are well known hallmarks of many human solid tumors and hematological disorders, where patient- and breakpoint-specific information may facilitate prognostication and individualized therapy. In thyroid carcinomas, the proto-oncogenes RET and NTRK1 are often found to be activated through chromosomal rearrangements. However, many sporadic tumors and papillary thyroid carcinomas (PTCs) arising in patients with a history of exposure to elevated levels of ionizing irradiation do not carry these known abnormalities. We developed a rapid scheme to screen tumor cell metaphase spreads and identify candidate genes of tumorigenesis and neoplastic progression for subsequent functional studies. Using a series of overnight fluorescence in situ hybridization (FISH) experiments with pools comprised of bacterial artificial chromosome (BAC) clones, it now becomes possible to rapidly refine breakpoint maps and, within one week, progress from the low resolution Spectral Karyotyping (SKY) maps or Giemsa-banding (G-banding) karyotypes to fully integrated, high resolution physical maps including a list of candiate genes in the critical regions.
Keywords: Chernobyl; neoplastic disease; papillary thyroid cancer; translocation; molecular cytogenetics; breakpoint delineation; fluorescence in situ hybridization; bacterial artificial chromosomes
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MDPI and ACS Style

Weier, H.-U.; Ito, Y.; Kwan, J.; Smida, J.; Weier, J.F.; Hieber, L.; Lu, C.-M.; Lehmann, L.; Wang, M.; Kassabian, H.J.; Zeng, H.; O’Brien, B. Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer. Genes 2011, 2, 397-419.

AMA Style

Weier H-U, Ito Y, Kwan J, Smida J, Weier JF, Hieber L, Lu C-M, Lehmann L, Wang M, Kassabian HJ, Zeng H, O’Brien B. Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer. Genes. 2011; 2(3):397-419.

Chicago/Turabian Style

Weier, Heinz-Ulrich G.; Ito, Yuko; Kwan, Johnson; Smida, Jan; Weier, Jingly F.; Hieber, Ludwig; Lu, Chun-Mei; Lehmann, Lars; Wang, Mei; Kassabian, Haig J.; Zeng, Hui; O’Brien, Benjamin. 2011. "Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer." Genes 2, no. 3: 397-419.

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