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Next Generation Sequencing: Advances in Characterizing the Methylome
University of Missouri-Columbia School of Medicine, Ellis Fischel Cancer Center, Columbia, MO 65212, USA
Medical College of Georgia, Augusta, GA 30912, USA
* Author to whom correspondence should be addressed.
Received: 3 May 2010; in revised form: 22 June 2010 / Accepted: 28 June 2010 / Published: 1 July 2010
Abstract: Epigenetic modifications play an important role in lymphoid malignancies. This has been evidenced by the large body of work published using microarray technologies to generate methylation profiles for numerous types and subtypes of lymphoma and leukemia. These studies have shown the importance of defining the epigenome so that we can better understand the biology of lymphoma. Recent advances in DNA sequencing technology have transformed the landscape of epigenomic analysis as we now have the ability to characterize the genome-wide distribution of chromatin modifications and DNA methylation using next-generation sequencing. To take full advantage of the throughput of next-generation sequencing, there are many methodologies that have been developed and many more that are currently being developed. Choosing the appropriate methodology is fundamental to the outcome of next-generation sequencing studies. In this review, published technologies and methodologies applicable to studying the methylome are presented. In addition, progress towards defining the methylome in lymphoma is discussed and prospective directions that have been made possible as a result of next-generation sequencing technology. Finally, methodologies are introduced that have not yet been published but that are being explored in the pursuit of defining the lymphoma methylome.
Keywords: lymphoma; leukemia; next-generation sequencing; methylation; epigenome
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Taylor, K.H.; Shi, H.; Caldwell, C.W. Next Generation Sequencing: Advances in Characterizing the Methylome. Genes 2010, 1, 143-165.
Taylor KH, Shi H, Caldwell CW. Next Generation Sequencing: Advances in Characterizing the Methylome. Genes. 2010; 1(2):143-165.
Taylor, Kristen H.; Shi, Huidong; Caldwell, Charles W. 2010. "Next Generation Sequencing: Advances in Characterizing the Methylome." Genes 1, no. 2: 143-165.