Next Article in Journal / Special Issue
Staggered Chromosomal Hybrid Zones in the House Mouse: Relevance to Reticulate Evolution and Speciation
Previous Article in Journal
Next Generation Sequencing: Advances in Characterizing the Methylome
Previous Article in Special Issue
Population Genomics of Secondary Contact
Article Menu

Export Article

Open AccessReview
Genes 2010, 1(2), 166-192; doi:10.3390/genes1020166

Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation)

1
Department of Biology, University of Florida, Gainesville, Florida 32611, USA
2
School of Biological and Chemical Sciences, Queen Mary, University of London, UK
3
Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611, USA
*
Author to whom correspondence should be addressed.
Received: 18 May 2010 / Revised: 30 June 2010 / Accepted: 30 June 2010 / Published: 2 July 2010
(This article belongs to the Special Issue Reticulate Evolution)
View Full-Text   |   Download PDF [392 KB, uploaded 2 July 2010]   |  

Abstract

The convergence of distinct lineages upon interspecific hybridisation, including when accompanied by increases in ploidy (allopolyploidy), is a driving force in the origin of many plant species. In plant breeding too, both interspecific hybridisation and allopolyploidy are important because they facilitate introgression of alien DNA into breeding lines enabling the introduction of novel characters. Here we review how fluorescence in situ hybridisation (FISH) and genomic in situ hybridisation (GISH) have been applied to: 1) studies of interspecific hybridisation and polyploidy in nature, 2) analyses of phylogenetic relationships between species, 3) genetic mapping and 4) analysis of plant breeding materials. We also review how FISH is poised to take advantage of nextgeneration sequencing (NGS) technologies, helping the rapid characterisation of the repetitive fractions of a genome in natural populations and agricultural plants. View Full-Text
Keywords: cytogenetics; ISH; polyploidy cytogenetics; ISH; polyploidy
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chester, M.; Leitch, A.R.; Soltis, P.S.; Soltis, D.E. Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation). Genes 2010, 1, 166-192.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top