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Genes 2010, 1(2), 166-192; doi:10.3390/genes1020166

Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation)

Department of Biology, University of Florida, Gainesville, Florida 32611, USA
School of Biological and Chemical Sciences, Queen Mary, University of London, UK
Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611, USA
Author to whom correspondence should be addressed.
Received: 18 May 2010 / Revised: 30 June 2010 / Accepted: 30 June 2010 / Published: 2 July 2010
(This article belongs to the Special Issue Reticulate Evolution)
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The convergence of distinct lineages upon interspecific hybridisation, including when accompanied by increases in ploidy (allopolyploidy), is a driving force in the origin of many plant species. In plant breeding too, both interspecific hybridisation and allopolyploidy are important because they facilitate introgression of alien DNA into breeding lines enabling the introduction of novel characters. Here we review how fluorescence in situ hybridisation (FISH) and genomic in situ hybridisation (GISH) have been applied to: 1) studies of interspecific hybridisation and polyploidy in nature, 2) analyses of phylogenetic relationships between species, 3) genetic mapping and 4) analysis of plant breeding materials. We also review how FISH is poised to take advantage of nextgeneration sequencing (NGS) technologies, helping the rapid characterisation of the repetitive fractions of a genome in natural populations and agricultural plants. View Full-Text
Keywords: cytogenetics; ISH; polyploidy cytogenetics; ISH; polyploidy

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chester, M.; Leitch, A.R.; Soltis, P.S.; Soltis, D.E. Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation). Genes 2010, 1, 166-192.

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