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Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells
“G.P. Livanos and M. Simou Laboratories”, Evangelismos Hospital, Department of Critical Care and Pulmonary Services, School of Medicine, University of Athens, 3 Ploutarchou Street, 10675 Athens, Greece
Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, 26504 Patras, Greece
* Author to whom correspondence should be addressed.
Received: 30 December 2010; in revised form: 9 February 2011 / Accepted: 15 February 2011 / Published: 23 February 2011
(This article belongs to the Special Issue Lung Cancer
Abstract: Mastic oil from Pistacia lentiscus variation chia, a natural combination of bioactive terpenes, has been shown to exert anti-tumor growth effects against a broad spectrum of cancers including mouse Lewis lung adenocarcinomas (LLC). However, no studies have addressed its anti-metastatic actions. In this study, we showed that treatment of LLC cells with mastic oil within a range of non-toxic concentrations (0.01–0.04% v/v): (a) abrogated their Matrigel invasion and migration capabilities in transwell assays; (b) reduced the levels of secreted MMP-2; (c) restricted phorbol ester-induced actin remodeling and (d) limited the length of neo-vessel networks in tumor microenvironment in the model of chick embryo chorioallantoic membrane. Moreover, exposure of LLC and endothelial cells to mastic oil impaired their adhesive interactions in a co-culture assay and reduced the expression of key adhesion molecules by endothelial cells upon their stimulation with tumor necrosis factor-alpha. Overall, this study provides novel evidence supporting a multipotent role for mastic oil in prevention of crucial processes related to cancer metastasis.
Keywords: lung cancer; prevention; mastic plant essential oil; invasion; motility; adhesion; angiogenesis
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Loutrari, H.; Magkouta, S.; Papapetropoulos, A.; Roussos, C. Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells. Cancers 2011, 3, 789-801.
Loutrari H, Magkouta S, Papapetropoulos A, Roussos C. Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells. Cancers. 2011; 3(1):789-801.
Loutrari, Heleni; Magkouta, Sophia; Papapetropoulos, Andreas; Roussos, Charis. 2011. "Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells." Cancers 3, no. 1: 789-801.