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Micromachines 2017, 8(6), 172; doi:10.3390/mi8060172

Automatic and Selective Single Cell Manipulation in a Pressure-Driven Microfluidic Lab-On-Chip Device

Department of Marine Engineering, Dalian Maritime University, Dalian 116026, China
Department of Radiotherapy, Jiaozhao Central Hospital, Qingdao 266300, China
Department of Respiratory Medicine, The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editors: Aaron T. Ohta and Nam-Trung Nguyen
Received: 7 April 2017 / Revised: 9 May 2017 / Accepted: 16 May 2017 / Published: 1 June 2017
View Full-Text   |   Download PDF [1888 KB, uploaded 2 June 2017]   |  


A microfluidic lab-on-chip device was developed to automatically and selectively manipulate target cells at the single cell level. The device is composed of a microfluidic chip, mini solenoid valves with negative-pressurized soft tubes, and a LabView®-based data acquisition device. Once a target cell passes the resistive pulse sensing gate of the microfluidic chip, the solenoid valves are automatically actuated and open the negative-pressurized tubes placed at the ends of the collecting channels. As a result, the cell is transported to that collecting well. Numerical simulation shows that a 0.14 mm3 volume change of the soft tube can result in a 1.58 mm/s moving velocity of the sample solution. Experiments with single polystyrene particles and cancer cells samples were carried out to demonstrate the effectiveness of this method. Selectively manipulating a certain size of particles from a mixture solution was also achieved. Due to the very high pressure-driven flow switching, as many as 300 target cells per minute can be isolated from the sample solution and thus is particularly suitable for manipulating very rare target cells. The device is simple, automatic, and label-free and particularly suitable for isolating single cells off the chip one by one for downstream analysis. View Full-Text
Keywords: single cell manipulation; microfluidic chip; resistive pulse sensing; hydrodynamic isolation single cell manipulation; microfluidic chip; resistive pulse sensing; hydrodynamic isolation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Shen, Y.; Song, Z.; Yan, Y.; Song, Y.; Pan, X.; Wang, Q. Automatic and Selective Single Cell Manipulation in a Pressure-Driven Microfluidic Lab-On-Chip Device. Micromachines 2017, 8, 172.

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