Next Article in Journal
Tip-Based Nanofabrication for Scalable Manufacturing
Next Article in Special Issue
Automatic and Selective Single Cell Manipulation in a Pressure-Driven Microfluidic Lab-On-Chip Device
Previous Article in Journal
Size-Adjustable Microdroplets Generation Based on Microinjection
Previous Article in Special Issue
1600 Parallel Microchamber Microfluidic Device for Fast Sample Array Preparation Using the Immiscibility of Two Liquids
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Micromachines 2017, 8(3), 89; doi:10.3390/mi8030089

Investigation of the Mechanical Properties of the Human Osteosarcoma Cell at Different Cell Cycle Stages †

1
School of Mechatronics Engineering, University of Electronic Science and Technology of China, Chengdu 611731, Sichuan, China
2
Center for Robotics, University of Electronic Science and Technology of China, Chengdu 611731, Sichuan, China
3
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, Sichuan, China
4
School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, Hunan, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Yongxin Song, Junsheng Wang and Dongqing Li
Received: 3 February 2017 / Revised: 1 March 2017 / Accepted: 10 March 2017 / Published: 15 March 2017
View Full-Text   |   Download PDF [3005 KB, uploaded 15 March 2017]   |  

Abstract

The mechanical properties of a single cell play substantial roles in cell mitosis, differentiation, and carcinogenesis. According to the difference of elastic modulus between the benign cell and the tumor cell, it has been shown that the mechanical properties of cells, as special biomarkers, may contribute greatly to disease diagnosis and drug screening. However, the mechanical properties of cells at different cell cycle stages are still not clear, which may mislead us when we use them as biomarkers. In this paper, the target regions of the human osteosarcoma cell were precisely scanned without causing any cell damage by using an atomic force microscopy (AFM) for the first time. Then, the elasticity properties of the human osteosarcoma cells were investigated quantitatively at various regions and cell cycle stages. The 32 × 32 resolution map of the elasticity showed that the elastic modulus of the cells at the interphase was larger than that at the telophase of mitosis. Moreover, the elastic modulus of the cell in the peripheral region was larger than that in the nuclear region of the cell. This work provides an accurate approach to measure the elasticity properties of cells at different stages of the cell cycle for further application in the disease diagnosis. View Full-Text
Keywords: mechanical property; elastic modulus; atomic force microscopy; human osteosarcoma cells; cell cycle mechanical property; elastic modulus; atomic force microscopy; human osteosarcoma cells; cell cycle
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhang, G.; Fan, N.; Lv, X.; Liu, Y.; Guo, J.; Yang, L.; Peng, B.; Jiang, H. Investigation of the Mechanical Properties of the Human Osteosarcoma Cell at Different Cell Cycle Stages †. Micromachines 2017, 8, 89.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top