Next Article in Journal
Resonance Spectrum Characteristics of Effective Electromechanical Coupling Coefficient of High-Overtone Bulk Acoustic Resonator
Next Article in Special Issue
A Study on the Influence of the Nozzle Lead Angle on the Performance of Liquid Metal Electromagnetic Micro-Jetting
Previous Article in Journal
Neural Circuits on a Chip
Previous Article in Special Issue
Integration of a Droplet-Based Microfluidic System and Silicon Nanoribbon FET Sensor
Article Menu

Export Article

Open AccessArticle
Micromachines 2016, 7(9), 161; doi:10.3390/mi7090161

Operation of Droplet-Microfluidic Devices with a Lab Centrifuge

Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences (QB3), University of California, San Francisco, CA 94115, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Andrew J. deMello and Xavier Casadevall i Solvas
Received: 7 June 2016 / Revised: 12 August 2016 / Accepted: 18 August 2016 / Published: 6 September 2016
(This article belongs to the Special Issue Droplet Microfluidics: Techniques and Technologies, Volume II)
View Full-Text   |   Download PDF [2336 KB, uploaded 6 September 2016]   |  

Abstract

Microfluidic devices are valuable for a variety of biotechnology applications, such as synthesizing biochemical libraries, screening enzymes, and analyzing single cells. However, normally, the devices are controlled using specialized pumps, which require expert knowledge to operate. Here, we demonstrate operation of poly(dimethylsiloxane) devices without pumps. We build a scaffold that holds the device and reagents to be infused in a format that can be inserted into a 50 mL falcon tube and spun in a common lab centrifuge. By controlling the device design and centrifuge spin speed, we infuse the reagents at controlled flow rates. We demonstrate the encapsulation and culture of clonal colonies of red and green Escherichia coli in droplets seeded from single cells. View Full-Text
Keywords: microfluidics; droplets; poly(dimethylsiloxane); lab on chip microfluidics; droplets; poly(dimethylsiloxane); lab on chip
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ahmed, N.; Sukovich, D.; Abate, A.R. Operation of Droplet-Microfluidic Devices with a Lab Centrifuge. Micromachines 2016, 7, 161.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top