A Microchip for High-Throughput Axon Growth Drug Screening
Abstract
:1. Introduction
2. Materials and Methods
2.1. Design
2.2. Fabrication
2.3. Tissue Dissociation and Cell Preparation
2.4. Microchip Cell Culture
2.5. Localized Biomolecular Treatment
2.6. Imaging
2.7. Fluidic Simulation
3. Results and Discussion
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
CNS | Central nervous system |
PNS | Peripheral nervous system |
MAG | Myelin-associated glycoprotein |
OMgp | Oligodendrocyte myelin glycoprotein |
PDMS | Poly(dimethylsiloxane) |
CNC | Computer numerically controlled |
PMMA | Poly(methyl methacrylate) |
AM | Acetoxymethyl |
SEM | Scanning electron microscope |
IPA | Isopropyl alcohol |
DI water | Deionized water |
E16 | Embryonic day 16 |
DIV | Days in vitro |
CSPG | Chondroitin sulfate proteoglycan |
PBS | Phosphate buffered saline |
FEM | Finite element method |
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Kim, H.S.; Jeong, S.; Koo, C.; Han, A.; Park, J. A Microchip for High-Throughput Axon Growth Drug Screening. Micromachines 2016, 7, 114. https://doi.org/10.3390/mi7070114
Kim HS, Jeong S, Koo C, Han A, Park J. A Microchip for High-Throughput Axon Growth Drug Screening. Micromachines. 2016; 7(7):114. https://doi.org/10.3390/mi7070114
Chicago/Turabian StyleKim, Hyun Soo, Sehoon Jeong, Chiwan Koo, Arum Han, and Jaewon Park. 2016. "A Microchip for High-Throughput Axon Growth Drug Screening" Micromachines 7, no. 7: 114. https://doi.org/10.3390/mi7070114