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Micromachines 2016, 7(7), 114; doi:10.3390/mi7070114

A Microchip for High-Throughput Axon Growth Drug Screening

1
Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA
2
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA
3
Department of Electronics and Control Engineering, Hanbat National University, Daejeon 305-719, Korea
4
Department of Electrical and Electronic Engineering, Neural and Cognitive Sciences Research Center, Southern University of Science and Technology, Shenzhen 518055, China
*
Author to whom correspondence should be addressed.
Academic Editors: Nam-Trung Nguyen and Seyed Ali Mousavi Shaegh
Received: 2 June 2016 / Revised: 24 June 2016 / Accepted: 28 June 2016 / Published: 7 July 2016
View Full-Text   |   Download PDF [7860 KB, uploaded 7 July 2016]   |  

Abstract

It has been recently known that not only the presence of inhibitory molecules associated with myelin but also the reduced growth capability of the axons limit mature central nervous system (CNS) axonal regeneration after injury. Conventional axon growth studies are typically conducted using multi-well cell culture plates that are very difficult to use for investigating localized effects of drugs and limited to low throughput. Unfortunately, there is currently no other in vitro tool that allows investigating localized axonal responses to biomolecules in high-throughput for screening potential drugs that might promote axonal growth. We have developed a compartmentalized neuron culture platform enabling localized biomolecular treatments in parallel to axons that are physically and fluidically isolated from their neuronal somata. The 24 axon compartments in the developed platform are designed to perform four sets of six different localized biomolecular treatments simultaneously on a single device. In addition, the novel microfluidic configuration allows culture medium of 24 axon compartments to be replenished altogether by a single aspiration process, making high-throughput drug screening a reality. View Full-Text
Keywords: microfluidic neuron culture platform; compartmentalized culture; localized biomolecular treatment; high-throughput screening microfluidic neuron culture platform; compartmentalized culture; localized biomolecular treatment; high-throughput screening
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MDPI and ACS Style

Kim, H.S.; Jeong, S.; Koo, C.; Han, A.; Park, J. A Microchip for High-Throughput Axon Growth Drug Screening. Micromachines 2016, 7, 114.

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