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Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
Department of Physics, University of Gothenburg, Gothenburg, SE-412 96, Sweden
* Author to whom correspondence should be addressed.
Received: 31 August 2013; in revised form: 15 November 2013 / Accepted: 25 November 2013 / Published: 3 December 2013
Abstract: The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min−1, moderate = 50 nL min−1, and high = 100 nL min−1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.
Keywords: microfluidics; single cell; high-throughput; hydrodynamic trapping; yeast; arsenite; PDMS; fluorescence microscopy
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MDPI and ACS Style
Banaeiyan, A.A.; Ahmadpour, D.; Adiels, C.B.; Goksör, M. Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications. Micromachines 2013, 4, 414-430.
Banaeiyan AA, Ahmadpour D, Adiels CB, Goksör M. Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications. Micromachines. 2013; 4(4):414-430.
Banaeiyan, Amin A.; Ahmadpour, Doryaneh; Adiels, Caroline B.; Goksör, Mattias. 2013. "Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications." Micromachines 4, no. 4: 414-430.