Micromachines 2013, 4(4), 414-430; doi:10.3390/mi4040414
Article

Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications

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Received: 31 August 2013; in revised form: 15 November 2013 / Accepted: 25 November 2013 / Published: 3 December 2013
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min−1, moderate = 50 nL min−1, and high = 100 nL min−1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.
Keywords: microfluidics; single cell; high-throughput; hydrodynamic trapping; yeast; arsenite; PDMS; fluorescence microscopy
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MDPI and ACS Style

Banaeiyan, A.A.; Ahmadpour, D.; Adiels, C.B.; Goksör, M. Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications. Micromachines 2013, 4, 414-430.

AMA Style

Banaeiyan AA, Ahmadpour D, Adiels CB, Goksör M. Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications. Micromachines. 2013; 4(4):414-430.

Chicago/Turabian Style

Banaeiyan, Amin A.; Ahmadpour, Doryaneh; Adiels, Caroline B.; Goksör, Mattias. 2013. "Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications." Micromachines 4, no. 4: 414-430.

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