Micromachines 2013, 4(4), 402-413; doi:10.3390/mi4040402
Communication

Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry

1,2email, 3email, 4email, 1,* email, 2,* email and 3,5,* email
Received: 5 September 2013; in revised form: 5 November 2013 / Accepted: 19 November 2013 / Published: 3 December 2013
(This article belongs to the Special Issue Lab-on-a-Chip)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.
Keywords: microfluidics; double emulsion; double flow-focusing geometry; flow cytometry
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MDPI and ACS Style

Yan, J.; Bauer, W.-A.C.; Fischlechner, M.; Hollfelder, F.; Kaminski, C.F.; Huck, W.T.S. Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry. Micromachines 2013, 4, 402-413.

AMA Style

Yan J, Bauer W-AC, Fischlechner M, Hollfelder F, Kaminski CF, Huck WTS. Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry. Micromachines. 2013; 4(4):402-413.

Chicago/Turabian Style

Yan, Jing; Bauer, Wolfgang-Andreas C.; Fischlechner, Martin; Hollfelder, Florian; Kaminski, Clemens F.; Huck, Wilhelm T.S. 2013. "Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry." Micromachines 4, no. 4: 402-413.

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