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Micromachines 2013, 4(4), 402-413; doi:10.3390/mi4040402
Communication

Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry

1,2
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3
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4
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1,* , 2,*  and 3,5,*
1 Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK 2 Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge CB2 3RA, UK 3 Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK 4 Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK 5 Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen 6525 AJ, The Netherlands
* Authors to whom correspondence should be addressed.
Received: 5 September 2013 / Revised: 5 November 2013 / Accepted: 19 November 2013 / Published: 3 December 2013
(This article belongs to the collection Lab-on-a-Chip)
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Abstract

Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.
Keywords: microfluidics; double emulsion; double flow-focusing geometry; flow cytometry microfluidics; double emulsion; double flow-focusing geometry; flow cytometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yan, J.; Bauer, W.-A.C.; Fischlechner, M.; Hollfelder, F.; Kaminski, C.F.; Huck, W.T.S. Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry. Micromachines 2013, 4, 402-413.

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