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Micromachines 2013, 4(2), 272-285; doi:10.3390/mi4020272

On-Chip Enucleation of Bovine Oocytes using Microrobot-Assisted Flow-Speed Control

1
Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
2
Department of Aerospace and Mechanical Engineering, University of California, Los Angeles, CA 90095, USA
*
Author to whom correspondence should be addressed.
Received: 1 April 2013 / Revised: 1 June 2013 / Accepted: 6 June 2013 / Published: 21 June 2013
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis)
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Abstract

In this study, we developed a microfluidic chip with a magnetically driven microrobot for oocyte enucleation. A microfluidic system was specially designed for enucleation, and the microrobot actively controls the local flow-speed distribution in the microfluidic chip. The microrobot can adjust fluid resistances in a channel and can open or close the channel to control the flow distribution. Analytical modeling was conducted to control the fluid speed distribution using the microrobot, and the model was experimentally validated. The novelties of the developed microfluidic system are as follows: (1) the cutting speed improved significantly owing to the local fluid flow control; (2) the cutting volume of the oocyte can be adjusted so that the oocyte undergoes less damage; and (3) the nucleus can be removed properly using the combination of a microrobot and hydrodynamic forces. Using this device, we achieved a minimally invasive enucleation process. The average enucleation time was 2.5 s and the average removal volume ratio was 20%. The proposed new system has the advantages of better operation speed, greater cutting precision, and potential for repeatable enucleation.
Keywords: magnetically driven microtool; oocyte enucleation; microfluidic chip magnetically driven microtool; oocyte enucleation; microfluidic chip
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Feng, L.; Hagiwara, M.; Ichikawa, A.; Arai, F. On-Chip Enucleation of Bovine Oocytes using Microrobot-Assisted Flow-Speed Control. Micromachines 2013, 4, 272-285.

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