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Micromachines 2013, 4(2), 257-271; doi:10.3390/mi4020257
Article

Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay

1
, 1,* , 2
, 1
, 1
, 3
, 1
 and 4
1 Department of Electronic Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan 2 Department of Mechanical Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan 3 Department of Biotechnology, Chung Yuan Christian University, Zhongli 32023, Taiwan 4 Insititute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw 02-109, Poland
* Author to whom correspondence should be addressed.
Received: 12 March 2013 / Revised: 4 June 2013 / Accepted: 4 June 2013 / Published: 18 June 2013
(This article belongs to the Special Issue Bioinspired Microsensors and Micromachines)
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Abstract

This article presents design and testing of a microfluidic platform for immunoassay. The method is based on sandwiched ELISA, whereby the primary antibody is immobilized on nitrocelluose and, subsequently, magnetic beads are used as a label to detect the analyte. The chip takes approximately 2 h and 15 min to complete the assay. A Hall Effect sensor using 0.35-μm BioMEMS TSMC technology (Taiwan Semiconductor Manufacturing Company Bio-Micro-Electro-Mechanical Systems) was fabricated to sense the magnetic field from the beads. Furthermore, florescence detection and absorbance measurements from the chip demonstrate successful immunoassay on the chip. In addition, investigation also covers the Hall Effect simulations, mechanical modeling of the bead–protein complex, testing of the microfluidic platform with magnetic beads averaging 10 nm, and measurements with an inductor-based system.
Keywords: microfluidics; magnetic bead; immunoassay; Hall Effect; Bio-MEMS; bead–protein complex microfluidics; magnetic bead; immunoassay; Hall Effect; Bio-MEMS; bead–protein complex
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Bhalla, N.; Chung, D.W.Y.; Chang, Y.-J.; Uy, K.J.S.; Ye, Y.Y.; Chin, T.-Y.; Yang, H.C.; Pijanowska, D.G. Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay. Micromachines 2013, 4, 257-271.

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