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Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay
Department of Electronic Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan
Department of Mechanical Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan
Department of Biotechnology, Chung Yuan Christian University, Zhongli 32023, Taiwan
Insititute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw 02-109, Poland
* Author to whom correspondence should be addressed.
Received: 12 March 2013; in revised form: 4 June 2013 / Accepted: 4 June 2013 / Published: 18 June 2013
Abstract: This article presents design and testing of a microfluidic platform for immunoassay. The method is based on sandwiched ELISA, whereby the primary antibody is immobilized on nitrocelluose and, subsequently, magnetic beads are used as a label to detect the analyte. The chip takes approximately 2 h and 15 min to complete the assay. A Hall Effect sensor using 0.35-μm BioMEMS TSMC technology (Taiwan Semiconductor Manufacturing Company Bio-Micro-Electro-Mechanical Systems) was fabricated to sense the magnetic field from the beads. Furthermore, florescence detection and absorbance measurements from the chip demonstrate successful immunoassay on the chip. In addition, investigation also covers the Hall Effect simulations, mechanical modeling of the bead–protein complex, testing of the microfluidic platform with magnetic beads averaging 10 nm, and measurements with an inductor-based system.
Keywords: microfluidics; magnetic bead; immunoassay; Hall Effect; Bio-MEMS; bead–protein complex
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MDPI and ACS Style
Bhalla, N.; Chung, D.W.Y.; Chang, Y.-J.; Uy, K.J.S.; Ye, Y.Y.; Chin, T.-Y.; Yang, H.C.; Pijanowska, D.G. Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay. Micromachines 2013, 4, 257-271.
Bhalla N, Chung DWY, Chang Y-J, Uy KJS, Ye YY, Chin T-Y, Yang HC, Pijanowska DG. Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay. Micromachines. 2013; 4(2):257-271.
Bhalla, Nikhil; Chung, Danny W.Y.; Chang, Yaw-Jen; Uy, Kimberly J.S.; Ye, Yi Y.; Chin, Ting-Yu; Yang, Hao C.; Pijanowska, Dorota G. 2013. "Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay." Micromachines 4, no. 2: 257-271.