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Micromachines 2013, 4(2), 243-256; doi:10.3390/mi4020243
Article

Analysis of Electric Fields inside Microchannels and Single Cell Electrical Lysis with a Microfluidic Device

1,* , 2
 and 3
Received: 16 March 2013; in revised form: 13 May 2013 / Accepted: 28 May 2013 / Published: 7 June 2013
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis)
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Abstract: Analysis of electric fields generated inside the microchannels of a microfluidic device for electrical lysis of biological cells along with experimental verification are presented. Electrical lysis is the complete disintegration of cell membranes, due to a critical level of electric fields applied for a critical duration on a biological cell. Generating an electric field inside a microchannel of a microfluidic device has many advantages, including the efficient utilization of energy and low-current requirement. An ideal microchannel model was compared with a practical microchannel model using a finite element analysis tool that suggests that the overestimation error can be over 10%, from 2.5 mm or smaller, in the length of a microchannel. Two analytical forms are proposed to reduce this overestimation error. Experimental results showed that the high electric field is confined only inside the microchannel that is in agreement with the simulation results. Single cell electrical lysis was conducted with a fabricated microfluidic device. An average of 800 V for seven seconds across an 8 mm-long microchannel with the dimension of 100 μm × 20 μm was required for lysis, with electric fields exceeding 100 kV/m and consuming 300 mW.
Keywords: biomedical electronics; electric fields; electrical lysis; finite element analysis; microchannel; microfluidics biomedical electronics; electric fields; electrical lysis; finite element analysis; microchannel; microfluidics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Morshed, B.I.; Shams, M.; Mussivand, T. Analysis of Electric Fields inside Microchannels and Single Cell Electrical Lysis with a Microfluidic Device. Micromachines 2013, 4, 243-256.

AMA Style

Morshed BI, Shams M, Mussivand T. Analysis of Electric Fields inside Microchannels and Single Cell Electrical Lysis with a Microfluidic Device. Micromachines. 2013; 4(2):243-256.

Chicago/Turabian Style

Morshed, Bashir I.; Shams, Maitham; Mussivand, Tofy. 2013. "Analysis of Electric Fields inside Microchannels and Single Cell Electrical Lysis with a Microfluidic Device." Micromachines 4, no. 2: 243-256.


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