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Toxins 2014, 6(5), 1512-1525; doi:10.3390/toxins6051512
Article

Role of Fc in Antibody-Mediated Protection from Ricin Toxin

1,2,* , 1,3
, 1,†
, 1
, 1
 and 4,‡
Received: 4 February 2014; in revised form: 24 March 2014 / Accepted: 28 March 2014 / Published: 7 May 2014
(This article belongs to the Special Issue Toxin-Antibody Interactions)
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Abstract: We have studied the role of the antibody (Ab) Fc region in mediating protection from ricin toxicity. We compared the in vitro and in vivo effects of intact Ig and of Fab fragments derived from two different neutralizing Ab preparations, one monoclonal, the other polyclonal. Consistent results were obtained from each, showing little difference between Ig and Fab in terms of antigen binding and in vitro neutralization, but with relatively large differences in protection of animals. We also studied whether importing Ab into the cell by Fc receptors enhanced the intracellular neutralization of ricin toxin. We found that the imported Ab was found in the ER and Golgi, a compartment traversed by ricin, as it traffics through the cell, but intracellular Ab did not contribute to the neutralization of ricin. These results indicate that the Fc region of antibody is important for in vivo protection, although the mechanism of enhanced protection by intact Ig does not appear to operate at the single cell level. When using xenogeneic antibodies, the diminished immunogenicity of Fab/F(ab’)2 preparations should be balanced against possible loss of protective efficacy.
Keywords: ricin; monoclonal antibody; polyclonal antiserum; Fc region; Fc receptor; Fab; neutralization; in vivo protection ricin; monoclonal antibody; polyclonal antiserum; Fc region; Fc receptor; Fab; neutralization; in vivo protection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pincus, S.H.; Das, A.; Song, K.; Maresh, G.A.; Corti, M.; Berry, J. Role of Fc in Antibody-Mediated Protection from Ricin Toxin. Toxins 2014, 6, 1512-1525.

AMA Style

Pincus SH, Das A, Song K, Maresh GA, Corti M, Berry J. Role of Fc in Antibody-Mediated Protection from Ricin Toxin. Toxins. 2014; 6(5):1512-1525.

Chicago/Turabian Style

Pincus, Seth. H.; Das, Anushka; Song, Kejing; Maresh, Grace A.; Corti, Miriam; Berry, Jody. 2014. "Role of Fc in Antibody-Mediated Protection from Ricin Toxin." Toxins 6, no. 5: 1512-1525.


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