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Toxins 2010, 2(8), 2007-2027; doi:10.3390/toxins2082007
Article

Unexpected Modulation of Recall B and T Cell Responses after Immunization with Rotavirus-like Particles in the Presence of LT-R192G

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Received: 13 July 2010; Accepted: 3 August 2010 / Published: 5 August 2010
(This article belongs to the Special Issue Enterotoxins)
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Abstract: LT-R192G, a mutant of the thermolabile enterotoxin of E. coli, is a potent adjuvant of immunization. Immune responses are generally analyzed at the end of protocols including at least 2 administrations, but rarely after a prime. To investigate this point, we compared B and T cell responses in mice after one and two intrarectal immunizations with 2/6 rotavirus-like particles (2/6-VLP) and LT-R192G. After a boost, we found, an unexpected lower B cell expansion measured by flow cytometry, despite a secondary antibody response. We then analyzed CD4+CD25+Foxp3+ regulatory T cells (Tregs) and CD4+CD25+Foxp3 helper T cells after in vitro (re)stimulation of mesenteric lymph node cells with the antigen (2/6-VLP), the adjuvant (LT-R192G) or both. 2/6-VLP did not activate CD4+CD25+Foxp3 nor Foxp3+ T cells from non-immunized and 2/6-VLP immunized mice, whereas they did activate both subsets from mice immunized with 2/6-VLP in the presence of adjuvant. LT-R192G dramatically decreased CD4+CD25+Foxp3+ T cells from non-immunized and 2/6-VLP immunized mice but not from mice immunized with 2/6-VLP and adjuvant. Moreover, in this case, LT-R192G increased Foxp3 expression on CD4+CD25+Foxp3+ cells, suggesting specific Treg activation during the recall. Finally, when both 2/6-VLP and LT-R192G were used for restimulation, LT-R192G clearly suppressed both 2/6-VLP-specific CD4+CD25+Foxp3 and Foxp3+ T cells. All together, these results suggest that LT-R192G exerts different effects on CD4+CD25+Foxp3+ T cells, depending on a first or a second contact. The unexpected immunomodulation observed during the recall should be considered in designing vaccination protocols.
Keywords: LT-R192G; Foxp3; CD25; regulatory T cells; B lymphocyte; B-1a lymphocyte; mucosal immunization; rotavirus LT-R192G; Foxp3; CD25; regulatory T cells; B lymphocyte; B-1a lymphocyte; mucosal immunization; rotavirus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Thiam, F.; Martino, C.D.; Bon, F.; Charpilienne, A.; Cachia, C.; Poncet, D.; Clements, J.D.; Basset, C.; Kohli, E. Unexpected Modulation of Recall B and T Cell Responses after Immunization with Rotavirus-like Particles in the Presence of LT-R192G. Toxins 2010, 2, 2007-2027.

AMA Style

Thiam F, Martino CD, Bon F, Charpilienne A, Cachia C, Poncet D, Clements JD, Basset C, Kohli E. Unexpected Modulation of Recall B and T Cell Responses after Immunization with Rotavirus-like Particles in the Presence of LT-R192G. Toxins. 2010; 2(8):2007-2027.

Chicago/Turabian Style

Thiam, Fatou; Martino, Cyrille Di; Bon, Fabienne; Charpilienne, Annie; Cachia, Claire; Poncet, Didier; Clements, John D.; Basset, Christelle; Kohli, Evelyne. 2010. "Unexpected Modulation of Recall B and T Cell Responses after Immunization with Rotavirus-like Particles in the Presence of LT-R192G." Toxins 2, no. 8: 2007-2027.



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