Nutrients 2013, 5(8), 3311-3328; doi:10.3390/nu5083311
Article

Dietary Fructose Feeding Increases Adipose Methylglyoxal Accumulation in Rats in Association with Low Expression and Activity of Glyoxalase-2

1,2email, 1email, 1email, 3email, 1email and 1,4,* email
Received: 9 June 2013; in revised form: 5 July 2013 / Accepted: 12 August 2013 / Published: 21 August 2013
(This article belongs to the Special Issue Nutritional Toxicology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Methylglyoxal is a precursor to advanced glycation endproducts that may contribute to diabetes and its cardiovascular-related complications. Methylglyoxal is successively catabolized to d-lactate by glyoxalase-1 and glyoxalase-2. The objective of this study was to determine whether dietary fructose and green tea extract (GTE) differentially regulate methylglyoxal accumulation in liver and adipose, mediated by tissue-specific differences in the glyoxalase system. We fed six week old male Sprague-Dawley rats a low-fructose diet (10% w/w) or a high-fructose diet (60% w/w) containing no GTE or GTE at 0.5% or 1.0% for nine weeks. Fructose-fed rats had higher (P < 0.05) adipose methylglyoxal, but GTE had no effect. Plasma and hepatic methylglyoxal were unaffected by fructose and GTE. Fructose and GTE also had no effect on the expression or activity of glyoxalase-1 and glyoxalase-2 at liver or adipose. Regardless of diet, adipose glyoxalase-2 activity was 10.8-times lower (P < 0.05) than adipose glyoxalase-1 activity and 5.9-times lower than liver glyoxalase-2 activity. Adipose glyoxalase-2 activity was also inversely related to adipose methylglyoxal (r = −0.61; P < 0.05). These findings suggest that fructose-mediated adipose methylglyoxal accumulation is independent of GTE supplementation and that its preferential accumulation in adipose compared to liver is due to low constitutive expression of glyoxalase-2.
Keywords: fructose; glyoxalase I; glyoxalase II; pyruvaldehyde; rats; Sprague-Dawley
PDF Full-text Download PDF Full-Text [640 KB, uploaded 21 August 2013 17:41 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Masterjohn, C.; Park, Y.; Lee, J.; Noh, S.K.; Koo, S.I.; Bruno, R.S. Dietary Fructose Feeding Increases Adipose Methylglyoxal Accumulation in Rats in Association with Low Expression and Activity of Glyoxalase-2. Nutrients 2013, 5, 3311-3328.

AMA Style

Masterjohn C, Park Y, Lee J, Noh SK, Koo SI, Bruno RS. Dietary Fructose Feeding Increases Adipose Methylglyoxal Accumulation in Rats in Association with Low Expression and Activity of Glyoxalase-2. Nutrients. 2013; 5(8):3311-3328.

Chicago/Turabian Style

Masterjohn, Christopher; Park, Youngki; Lee, Jiyoung; Noh, Sang K.; Koo, Sung I.; Bruno, Richard S. 2013. "Dietary Fructose Feeding Increases Adipose Methylglyoxal Accumulation in Rats in Association with Low Expression and Activity of Glyoxalase-2." Nutrients 5, no. 8: 3311-3328.


Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert