Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Nutrients 2013, 5(7), 2667-2683; doi:10.3390/nu5072667
Article

Effects of cis-9,trans-11 and trans-10,cis-12 Conjugated Linoleic Acid, Linoleic Acid, Phytanic Acid and the Combination of Various Fatty Acids on Proliferation and Cytokine Expression of Bovine Peripheral Blood Mononuclear Cells

1
, 1,* , 2
, 2
 and 1
Received: 8 April 2013; in revised form: 31 May 2013 / Accepted: 24 June 2013 / Published: 12 July 2013
(This article belongs to the Special Issue Nutritional Toxicology)
View Full-Text   |   Download PDF [258 KB, uploaded 12 July 2013]
Abstract: Fatty acids may have an impact on immune functions, which is important in times of increased mobilization of body fat, e.g., around parturition. The aim of the present study was to investigate the effects of the CLA isomers cis-9,trans-11 and trans-10,cis-12, phytanic acid (PA), linoleic acid (LA) and a fatty acid (FA) mixture (containing 29.8% palmitic acid, 6.7% palmitoleic acid, 17.4% stearic acid and 46.1% oleic acid) on the proliferation of bovine blood mononuclear cells (PBMC) in vitro using alamar blue (AB) and 5-bromo-2′-deoxyuridine (BrdU) assay. Quantitative real time polymerase chain reaction analyses were performed to evaluate the expression of interleukin (IL)-4, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α and peroxisome proliferator-activated receptor (PPAR)-γ in response to cis-9,trans-11 and LA. The IC50 values did not differ between the investigated FA, but there were differences within the proliferation in the response of these FA in a concentration range between 20 and 148 µM (e.g., increased proliferation after treatment with lower concentrations of LA). No differences occurred when different FA combinations were tested. ConA stimulation increased the expression of TNF-α and IFN-γ, whereas IL-10 decreased. In general, neither the baseline expression nor the ConA-stimulated mRNA expression of cytokines and PPAR-γ were affected by the FA. In conclusion, all FA inhibit the proliferation of PBMC dose dependently without significantly altering the induced cytokine spectrum of activated bovine PBMC.
Keywords: conjugated linoleic acid; phytanic acid; bovine peripheral blood mononuclear cells; proliferation; cytokine expression conjugated linoleic acid; phytanic acid; bovine peripheral blood mononuclear cells; proliferation; cytokine expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Renner, L.; Kersten, S.; Duevel, A.; Schuberth, H.-J.; Dänicke, S. Effects of cis-9,trans-11 and trans-10,cis-12 Conjugated Linoleic Acid, Linoleic Acid, Phytanic Acid and the Combination of Various Fatty Acids on Proliferation and Cytokine Expression of Bovine Peripheral Blood Mononuclear Cells. Nutrients 2013, 5, 2667-2683.

AMA Style

Renner L, Kersten S, Duevel A, Schuberth H-J, Dänicke S. Effects of cis-9,trans-11 and trans-10,cis-12 Conjugated Linoleic Acid, Linoleic Acid, Phytanic Acid and the Combination of Various Fatty Acids on Proliferation and Cytokine Expression of Bovine Peripheral Blood Mononuclear Cells. Nutrients. 2013; 5(7):2667-2683.

Chicago/Turabian Style

Renner, Lydia; Kersten, Susanne; Duevel, Anna; Schuberth, Hans-Joachim; Dänicke, Sven. 2013. "Effects of cis-9,trans-11 and trans-10,cis-12 Conjugated Linoleic Acid, Linoleic Acid, Phytanic Acid and the Combination of Various Fatty Acids on Proliferation and Cytokine Expression of Bovine Peripheral Blood Mononuclear Cells." Nutrients 5, no. 7: 2667-2683.



Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert