Next Article in Journal
Molecular Genetic Analysis of Orf Virus: A Poxvirus That Has Adapted to Skin
Next Article in Special Issue
Wolbachia Do Not Induce Reactive Oxygen Species-Dependent Immune Pathway Activation in Aedes albopictus
Previous Article in Journal
Chondroitin Sulfate N-acetylgalactosaminyltransferase-2 Contributes to the Replication of Infectious Bursal Disease Virus via Interaction with the Capsid Protein VP2
Previous Article in Special Issue
Genetic Characterization of Goutanap Virus, a Novel Virus Related to Negeviruses, Cileviruses and Higreviruses
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(3), 1492-1504; doi:10.3390/v7031492

Facilitation of Rice Stripe Virus Accumulation in the Insect Vector by Himetobi P Virus VP1

1,2,†
,
1,†
,
2
,
2
,
1,* and 2,*
1
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
2
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Nikolaos Vasilakis
Received: 14 December 2014 / Revised: 3 March 2015 / Accepted: 17 March 2015 / Published: 23 March 2015
(This article belongs to the Special Issue Impact of the Insect Microbiome on Arbovirus Transmission)
View Full-Text   |   Download PDF [596 KB, uploaded 12 May 2015]   |  

Abstract

The small brown planthopper (SBPH) is the main vector for rice stripe virus (RSV), which causes serious rice stripe disease in East Asia. To characterize the virus-vector interactions, the SBPH cDNA library was screened with RSV ribonucleoprotein (RNP) as bait using a GAL4-based yeast two-hybrid system. The interaction between RSV-RNP and the Himetobi P virus (HiPV, an insect picorna-like virus) VP1 protein was identified. The relationships between HiPV and RSV in SBPH were further investigated, and the results showed that the titer of RSV was commonly higher in single insect that exhibited more VP1 expression. After the VP1 gene was repressed by RNA silencing, the accumulation of RSV decreased significantly in the insect, whereas the virus acquisition ability of SBPH was unaffected, which suggests that HiPV VP1 potentially facilitates the accumulation of RSV in SBPH. View Full-Text
Keywords: rice stripe virus; Himetobi P virus; small brown planthopper; accumulation of RSV rice stripe virus; Himetobi P virus; small brown planthopper; accumulation of RSV
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, S.; Ge, S.; Wang, X.; Sun, L.; Liu, Z.; Zhou, Y. Facilitation of Rice Stripe Virus Accumulation in the Insect Vector by Himetobi P Virus VP1. Viruses 2015, 7, 1492-1504.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top