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Viruses 2015, 7(3), 1474-1491; doi:10.3390/v7031474

Chondroitin Sulfate N-acetylgalactosaminyltransferase-2 Contributes to the Replication of Infectious Bursal Disease Virus via Interaction with the Capsid Protein VP2

1
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China
2
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 9 January 2015 / Revised: 13 March 2015 / Accepted: 16 March 2015 / Published: 23 March 2015
(This article belongs to the Section Animal Viruses)
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Abstract

Infectious bursal disease virus (IBDV) is a birnavirus that causes a highly contagious immunosuppressive disease in young chickens. The capsid protein VP2 of IBDV plays multiple roles in its life cycle. To more comprehensively understand the functions of VP2 involved in the communication between virus and host, we used yeast two-hybrid screening to identify the cellular factors that interact with this protein. We found that chondroitin sulfate N-acetylgalactosaminyltransferase-2 (CSGalNAcT2), a typical type II transmembrane protein located in Golgi apparatus, could interact with VP2, and we confirmed this interaction by co-immunoprecipitation and confocal laser scanning microscopy assays. Additionally, up-regulation of CSGalNAcT2 during IBDV infection was observed. Overexpression and siRNA-mediated knockdown of CSGalNAcT2 assays suggested that CSGalNAcT2 promoted IBDV replication. Moreover, this enhancing effect of CSGalNAcT2 could be inhibited by Brefeldin A, which is a Golgi-disturbing agent. This indicated that the integrity of Golgi apparatus structure was involved in the function of CSGalNAcT2. Taken together, we concluded that CSGalNAcT2, located in the Golgi apparatus, contributed to the replication of IBDV via interaction with VP2. View Full-Text
Keywords: CSGalNAcT2; Golgi apparatus; infectious bursal disease virus; growth; VP2 CSGalNAcT2; Golgi apparatus; infectious bursal disease virus; growth; VP2
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhang, L.; Ren, X.; Chen, Y.; Gao, Y.; Wang, N.; Lu, Z.; Gao, L.; Qin, L.; Wang, Y.; Gao, H.; Li, K.; Jiang, L.; Cui, H.; Liu, C.; Zhang, Y.; Qi, X.; Wang, X. Chondroitin Sulfate N-acetylgalactosaminyltransferase-2 Contributes to the Replication of Infectious Bursal Disease Virus via Interaction with the Capsid Protein VP2. Viruses 2015, 7, 1474-1491.

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