Viruses 2013, 5(7), 1802-1814; doi:10.3390/v5071802
Article

Deletions within the 3' Non-Translated Region of Alfalfa mosaic virus RNA4 Do Not Affect Replication but Significantly Reduce Long-Distance Movement of Chimeric Tobacco mosaic virus

Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Newark, DE 19711, USA
* Author to whom correspondence should be addressed.
Received: 3 May 2013; in revised form: 25 June 2013 / Accepted: 4 July 2013 / Published: 17 July 2013
(This article belongs to the Special Issue Plant Viruses)
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Abstract: Alfalfa mosaic virus (AlMV) RNAs 1 and 2 with deletions in their 3' non‑translated regions (NTRs) have been previously shown to be encapsidated into virions by coat protein (CP) expressed from RNA3, indicating that the 3' NTRs of RNAs 1 and 2 are not required for virion assembly. Here, we constructed various mutants by deleting sequences within the 3' NTR of AlMV subgenomic (sg) RNA4 (same as of RNA3) and examined the effect of these deletions on replication and translation of chimeric Tobacco mosaic virus (TMV) expressing AlMV sgRNA4 from the TMV CP sg promoter (Av/A4) in tobacco protoplasts and Nicotiana benthamiana plants. While the Av/A4 mutants were as competent as the wild-type Av/A4 in RNA replication in protoplasts, their encapsidation, long-distance movement and virus accumulation varied significantly in N. benthamiana. These data suggest that the 3' NTR of AlMV sgRNA4 contains potential elements necessary for virus encapsidation.
Keywords: Alfalfa mosaic virus; Tobacco mosaic virus; origin of assembly; subgenomic RNA; virus encapsidation

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MDPI and ACS Style

Roy, G.; Fedorkin, O.; Fujiki, M.; Skarjinskaia, M.; Knapp, E.; Rabindran, S.; Yusibov, V. Deletions within the 3' Non-Translated Region of Alfalfa mosaic virus RNA4 Do Not Affect Replication but Significantly Reduce Long-Distance Movement of Chimeric Tobacco mosaic virus. Viruses 2013, 5, 1802-1814.

AMA Style

Roy G, Fedorkin O, Fujiki M, Skarjinskaia M, Knapp E, Rabindran S, Yusibov V. Deletions within the 3' Non-Translated Region of Alfalfa mosaic virus RNA4 Do Not Affect Replication but Significantly Reduce Long-Distance Movement of Chimeric Tobacco mosaic virus. Viruses. 2013; 5(7):1802-1814.

Chicago/Turabian Style

Roy, Gourgopal; Fedorkin, Oleg; Fujiki, Masaaki; Skarjinskaia, Marina; Knapp, Elisabeth; Rabindran, Shailaja; Yusibov, Vidadi. 2013. "Deletions within the 3' Non-Translated Region of Alfalfa mosaic virus RNA4 Do Not Affect Replication but Significantly Reduce Long-Distance Movement of Chimeric Tobacco mosaic virus." Viruses 5, no. 7: 1802-1814.

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