Viruses 2013, 5(7), 1655-1663; doi:10.3390/v5071655
Article

Differentiation and Distribution of Cordyline Viruses 1–4 in Hawaiian ti Plants (Cordyline fruticosa L.)

1 Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI 96822, USA 2 Department of Plant and Environmental Protection Sciences, University of Hawaii, 45-260 Waikalua Road, Kaneohe, HI 96744, USA
* Author to whom correspondence should be addressed.
Received: 3 May 2013; in revised form: 15 June 2013 / Accepted: 26 June 2013 / Published: 5 July 2013
(This article belongs to the Special Issue Plant Viruses)
PDF Full-text Download PDF Full-Text [2557 KB, uploaded 5 July 2013 14:52 CEST]
Abstract: Common green ti plants (Cordyline fruticosa L.) in Hawaii can be infected by four recently characterized closteroviruses that are tentative members of the proposed genus Velarivirus. In this study, a reverse-transcription polymerase chain reaction (RT-PCR) assay developed to detect and distinguish Cordyline virus 1 (CoV-1), CoV-2, CoV-3, and CoV-4 was used to determine: (i) the distribution of these viruses in Hawaii; and (ii) if they are involved in the etiology of ti ringspot disease. One hundred and thirty-seven common green ti plants with and without ti ringspot symptoms were sampled from 43 sites on five of the Hawaiian Islands and underwent the RT-PCR assay. Eleven ornamental ti varieties were also sampled and assayed. Based on this survey, it appears none of the CoVs are involved in the etiology of ti ringspot. The observation of a non-uniform geographic distribution of the CoVs in common green ti, combined with the presence of CoVs in seed-derived ornamental varieties, suggests active vector transmission. Eight herbarium specimens collected between 1903 and 2003 from plants on the island of Oahu also underwent the RT-PCR assay. Amplifiable RNA was isolated from accessions collected in 1985 or later, however only the 2003 accession was found to harbor CoVs.
Keywords: cordyline virus; velarivirus; ti ringspot; herbarium sampling

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Melzer, M.; Ayin, C.; Sugano, J.; Uchida, J.; Kawate, M.; Borth, W.; Hu, J. Differentiation and Distribution of Cordyline Viruses 1–4 in Hawaiian ti Plants (Cordyline fruticosa L.). Viruses 2013, 5, 1655-1663.

AMA Style

Melzer M, Ayin C, Sugano J, Uchida J, Kawate M, Borth W, Hu J. Differentiation and Distribution of Cordyline Viruses 1–4 in Hawaiian ti Plants (Cordyline fruticosa L.). Viruses. 2013; 5(7):1655-1663.

Chicago/Turabian Style

Melzer, Michael; Ayin, Caleb; Sugano, Jari; Uchida, Janice; Kawate, Michael; Borth, Wayne; Hu, John. 2013. "Differentiation and Distribution of Cordyline Viruses 1–4 in Hawaiian ti Plants (Cordyline fruticosa L.)." Viruses 5, no. 7: 1655-1663.

Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert