Viruses 2013, 5(10), 2375-2383; doi:10.3390/v5102375
Brief Report

Sequence Heterogeneity of the ORF3 Gene of Porcine Epidemic Diarrhea Viruses Field Samples in Fujian, China, 2010–2012

1email, 2email, 1email, 1email, 1email, 2email, 1email and 1,* email
Received: 7 August 2013; in revised form: 13 September 2013 / Accepted: 19 September 2013 / Published: 30 September 2013
(This article belongs to the Special Issue Animal Arteriviruses and Coronaviruses)
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Abstract: Twenty-seven field samples that showed positive in PEDV detection were collected from different farms of Fujian province from 2010 to 2012. Their heterogeneity was investigated by analysis of the ORF3 gene because of its potential function as a representation of virulence. According to the results, six Fujian strains in Group 1 showed a different genotype with unique point mutations, which might be used in differentiation between PEDV groups and brought potential antigenic variation. P55 and five reference strains in Group 2 had a long length deletion, showing another genotype and might be involved in the variation of virulence. Phylogenetic analysis revealed that the collected Fujian strains were very distant from the vaccine development strain CV777, which might be the reason why the vaccine was inefficient to control the disease. The results can help to reconsider the strategy of PEDV vaccine management and prevent outbreaks of PEDV-induced diarrhea more efficiently.
Keywords: porcine epidemic diarrhea virus; PEDV; heterogeneity; phylogenetic analysis; vaccine development
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chen, X.; Zeng, L.; Yang, J.; Yu, F.; Ge, J.; Guo, Q.; Gao, X.; Song, T. Sequence Heterogeneity of the ORF3 Gene of Porcine Epidemic Diarrhea Viruses Field Samples in Fujian, China, 2010–2012. Viruses 2013, 5, 2375-2383.

AMA Style

Chen X, Zeng L, Yang J, Yu F, Ge J, Guo Q, Gao X, Song T. Sequence Heterogeneity of the ORF3 Gene of Porcine Epidemic Diarrhea Viruses Field Samples in Fujian, China, 2010–2012. Viruses. 2013; 5(10):2375-2383.

Chicago/Turabian Style

Chen, Xi; Zeng, Lili; Yang, Jinxian; Yu, Fusong; Ge, Junqing; Guo, Qing; Gao, Xindang; Song, Tieying. 2013. "Sequence Heterogeneity of the ORF3 Gene of Porcine Epidemic Diarrhea Viruses Field Samples in Fujian, China, 2010–2012." Viruses 5, no. 10: 2375-2383.


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