Viruses 2013, 5(8), 1991-2004; doi:10.3390/v5081991
Article

Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012

1 College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China 2 Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China 3 Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China 4 Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250132, China These authors contributed equally to this work. These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 17 June 2013; in revised form: 24 July 2013 / Accepted: 1 August 2013 / Published: 15 August 2013
(This article belongs to the Special Issue Animal Arteriviruses and Coronaviruses)
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Abstract: Between January 2012 and March 2012, the infection rates of porcine epidemic diarrhea virus (PEDV) increased substantially in vaccinated swine herds in many porcine farms in Gansu Province, China. The spike (S) glycoprotein is an important determinant for PEDV biological properties. To determine the distribution profile of PEDV outbreak strains, we sequenced the full-length S gene of five samples from two farms where animals exhibited severe diarrhea and high mortality rates. Five new PEDV variants were identified, and the molecular diversity, phylogenetic relationships, and antigenicity analysis of Gansu field samples with other PEDV reference strains were investigated. A series of insertions, deletions, and mutations in the S gene was found in five PEDV variants compared with classical and vaccine strains. These mutations may provide stronger pathogenicity and antigenicity to the new PEDV variants that influenced the effectiveness of the CV777-based vaccine. Our results suggest that these new PEDV variant strains in Gansu Province might be from South Korean or South China, and the effectiveness of the CV777-based vaccine needs to be evaluated.
Keywords: molecular characterization; spike glycoprotein gene; phylogenetic analysis; porcine epidemic diarrhea virus (PEDV)

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MDPI and ACS Style

Tian, Y.; Yu, Z.; Cheng, K.; Liu, Y.; Huang, J.; Xin, Y.; Li, Y.; Fan, S.; Wang, T.; Huang, G.; Feng, N.; Yang, Z.; Yang, S.; Gao, Y.; Xia, X. Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012. Viruses 2013, 5, 1991-2004.

AMA Style

Tian Y, Yu Z, Cheng K, Liu Y, Huang J, Xin Y, Li Y, Fan S, Wang T, Huang G, Feng N, Yang Z, Yang S, Gao Y, Xia X. Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012. Viruses. 2013; 5(8):1991-2004.

Chicago/Turabian Style

Tian, Yufei; Yu, Zhijun; Cheng, Kaihui; Liu, Yuxiu; Huang, Jing; Xin, Yue; Li, Yuanguo; Fan, Shengtao; Wang, Tiecheng; Huang, Geng; Feng, Na; Yang, Zhenguo; Yang, Songtao; Gao, Yuwei; Xia, Xianzhu. 2013. "Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012." Viruses 5, no. 8: 1991-2004.

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