Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China
AbstractSince late 2010, porcine epidemic diarrhea virus (PEDV) has rapidly disseminated all over the China and caused considerable morbidity and high mortality (up to 100%) in neonatal piglets. 79.66% (141 of 177) pig farms in 29 provinces (excluding Tibet and Hainan, China) and 72.27% (417 of 577) samples were positive for PEDV confirmed by reverse transcription-polymerase chain reaction (RT-PCR). The full-length S genes of representative field strains were sequenced. 33 field strains share 93.5%–99.9% homologies with each other at the nucleotide sequence level and 92.3%–99.8% homologies with each other at the amino acids sequence level. Most field strains have nucleotide deletion and insertion regions, and show lower homologies (93.5%–94.2%) with Chinese classical strain CH/S, however higher homologies (97.1%–99.3%) with recent strain CHGD-1. The phylogenetic analysis showed there are classical strains and variants prevailing in pig herd in China. PEDV has a high detection rate in pig herds in China. Sequence analysis indicated the S genes of recent field strains have heterogeneity and the variants are predominant.
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Chen, J.; Liu, X.; Shi, D.; Shi, H.; Zhang, X.; Li, C.; Chi, Y.; Feng, L. Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China. Viruses 2013, 5, 2601-2613.
Chen J, Liu X, Shi D, Shi H, Zhang X, Li C, Chi Y, Feng L. Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China. Viruses. 2013; 5(10):2601-2613.Chicago/Turabian Style
Chen, Jianfei; Liu, Xiaozhen; Shi, Da; Shi, Hongyan; Zhang, Xin; Li, Changlong; Chi, Yanbin; Feng, Li. 2013. "Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China." Viruses 5, no. 10: 2601-2613.