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Viruses 2013, 5(1), 54-86; doi:10.3390/v5010054
Review

Utility of the Bacteriophage RB69 Polymerase gp43 as a Surrogate Enzyme for Herpesvirus Orthologs

1 and 1,2,3,*
Received: 1 December 2012; in revised form: 16 December 2012 / Accepted: 17 December 2012 / Published: 8 January 2013
(This article belongs to the Special Issue Recent Progress in Bacteriophage Research)
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Abstract: Viral polymerases are important targets in drug discovery and development efforts. Most antiviral compounds that are currently approved for treatment of infection with members of the herpesviridae family were shown to inhibit the viral DNA polymerase. However, biochemical studies that shed light on mechanisms of drug action and resistance are hampered primarily due to technical problems associated with enzyme expression and purification. In contrast, the orthologous bacteriophage RB69 polymerase gp43 has been crystallized in various forms and therefore serves as a model system that provides a better understanding of structure–function relationships of polymerases that belong the type B family. This review aims to discuss strengths, limitations, and opportunities of the phage surrogate with emphasis placed on its utility in the discovery and development of anti-herpetic drugs.
Keywords: DNA polymerase; T4 DNA polymerase; gp43; herpesviridae; UL30; UL54; HSV1; HCMV; RB69 DNA polymerase DNA polymerase; T4 DNA polymerase; gp43; herpesviridae; UL30; UL54; HSV1; HCMV; RB69 DNA polymerase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Bennett, N.; Götte, M. Utility of the Bacteriophage RB69 Polymerase gp43 as a Surrogate Enzyme for Herpesvirus Orthologs. Viruses 2013, 5, 54-86.

AMA Style

Bennett N, Götte M. Utility of the Bacteriophage RB69 Polymerase gp43 as a Surrogate Enzyme for Herpesvirus Orthologs. Viruses. 2013; 5(1):54-86.

Chicago/Turabian Style

Bennett, Nicholas; Götte, Matthias. 2013. "Utility of the Bacteriophage RB69 Polymerase gp43 as a Surrogate Enzyme for Herpesvirus Orthologs." Viruses 5, no. 1: 54-86.


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