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A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections
Laboratory for Bacteriophages Genetics. Mechnikov Research Institute of Vaccines and Sera, RAMS, 5a, Maliy Kazenniy per., Moscow 105064, Russia
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Received: 10 November 2012; in revised form: 3 December 2012 / Accepted: 12 December 2012 / Published: 21 December 2012
Abstract: Pseudomonas aeruginosa is a frequent participant in wound infections. Emergence of multiple antibiotic resistant strains has created significant problems in the treatment of infected wounds. Phage therapy (PT) has been proposed as a possible alternative approach. Infected wounds are the perfect place for PT applications, since the basic condition for PT is ensured; namely, the direct contact of bacteria and their viruses. Plenty of virulent (“lytic”) and temperate (“lysogenic”) bacteriophages are known in P. aeruginosa. However, the number of virulent phage species acceptable for PT and their mutability are limited. Besides, there are different deviations in the behavior of virulent (and temperate) phages from their expected canonical models of development. We consider some examples of non-canonical phage-bacterium interactions and the possibility of their use in PT. In addition, some optimal approaches to the development of phage therapy will be discussed from the point of view of a biologist, considering the danger of phage-assisted horizontal gene transfer (HGT), and from the point of view of a surgeon who has accepted the Hippocrates Oath to cure patients by all possible means. It is also time now to discuss the possible approaches in international cooperation for the development of PT. We think it would be advantageous to make phage therapy a kind of personalized medicine.
Keywords: Pseudomonas aeruginosa bacteriophages diversity; noncanonical relations of phages and bacteria; phage’s migrations; phage genomes instability; pseudolysogeny and pseudovirulence; phage therapy as a kind of personalized medicine
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Krylov, V.; Shaburova, O.; Krylov, S.; Pleteneva, E. A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections. Viruses 2013, 5, 15-53.
Krylov V, Shaburova O, Krylov S, Pleteneva E. A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections. Viruses. 2013; 5(1):15-53.
Krylov, Victor; Shaburova, Olga; Krylov, Sergey; Pleteneva, Elena. 2013. "A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections." Viruses 5, no. 1: 15-53.