Next Article in Journal
Dendritic Cells and HIV-1 Trans-Infection
Next Article in Special Issue
Increasing the Efficacy of Oncolytic Adenovirus Vectors
Previous Article in Journal
HIV-1 Virological Synapse: Live Imaging of Transmission
Viruses 2010, 2(8), 1681-1703; doi:10.3390/v2081681

Adenoviral Producer Cells

VectorLogics, Inc., 550 11th Street South, Birmingham, AL35294, USA
* Author to whom correspondence should be addressed.
Received: 16 June 2010 / Revised: 30 July 2010 / Accepted: 13 August 2010 / Published: 16 August 2010
(This article belongs to the Special Issue Adenoviral Vectors)
View Full-Text   |   Download PDF [208 KB, uploaded 12 May 2015]   |   Browse Figures


Adenovirus (Ad) vectors, in particular those of the serotype 5, are highly attractive for a wide range of gene therapy, vaccine and virotherapy applications (as discussed in further detail in this issue). Wild type Ad5 virus can replicate in numerous tissue types but to use Ad vectors for therapeutic purposes the viral genome requires modification. In particular, if the viral genome is modified in such a way that the viral life cycle is interfered with, a specific producer cell line is required to provide trans-complementation to overcome the modification and allow viral production. This can occur in two ways; use of a producer cell line that contains specific adenoviral sequences incorporated into the cell genome to trans-complement, or use of a producer cell line that naturally complements for the modified Ad vector genome. This review concentrates on producer cell lines that complement non-replicating adenoviral vectors, starting with the historical HEK293 cell line developed in 1977 for first generation Ad vectors. In addition the problem of replication-competent adenovirus (RCA) contamination in viral preparations from HEK293 cells is addressed leading to the development of alternate cell lines. Furthermore novel cell lines for more complex Ad vectors and alternate serotype Ad vectors are discussed.
Keywords: producer cell lines; adenovirus; Ad vectors; RCA producer cell lines; adenovirus; Ad vectors; RCA
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Kovesdi, I.; Hedley, S.J. Adenoviral Producer Cells. Viruses 2010, 2, 1681-1703.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert