Viruses 2010, 2(8), 1681-1703; doi:10.3390/v2081681
Review

Adenoviral Producer Cells

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Received: 16 June 2010; in revised form: 30 July 2010 / Accepted: 13 August 2010 / Published: 16 August 2010
(This article belongs to the Special Issue Adenoviral Vectors)
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Abstract: Adenovirus (Ad) vectors, in particular those of the serotype 5, are highly attractive for a wide range of gene therapy, vaccine and virotherapy applications (as discussed in further detail in this issue). Wild type Ad5 virus can replicate in numerous tissue types but to use Ad vectors for therapeutic purposes the viral genome requires modification. In particular, if the viral genome is modified in such a way that the viral life cycle is interfered with, a specific producer cell line is required to provide trans-complementation to overcome the modification and allow viral production. This can occur in two ways; use of a producer cell line that contains specific adenoviral sequences incorporated into the cell genome to trans-complement, or use of a producer cell line that naturally complements for the modified Ad vector genome. This review concentrates on producer cell lines that complement non-replicating adenoviral vectors, starting with the historical HEK293 cell line developed in 1977 for first generation Ad vectors. In addition the problem of replication-competent adenovirus (RCA) contamination in viral preparations from HEK293 cells is addressed leading to the development of alternate cell lines. Furthermore novel cell lines for more complex Ad vectors and alternate serotype Ad vectors are discussed.
Keywords: producer cell lines; adenovirus; Ad vectors; RCA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kovesdi, I.; Hedley, S.J. Adenoviral Producer Cells. Viruses 2010, 2, 1681-1703.

AMA Style

Kovesdi I, Hedley SJ. Adenoviral Producer Cells. Viruses. 2010; 2(8):1681-1703.

Chicago/Turabian Style

Kovesdi, Imre; Hedley, Susan J. 2010. "Adenoviral Producer Cells." Viruses 2, no. 8: 1681-1703.


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