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Viruses 2010, 2(4), 939-960; doi:10.3390/v2040939
Review

Implications of the Nucleocapsid and the Microenvironment in Retroviral Reverse Transcription

1
, 2
 and 2,*
Received: 4 February 2010; in revised form: 3 March 2010 / Accepted: 1 April 2010 / Published: 2 April 2010
(This article belongs to the Special Issue Retroviral Enzymes)
View Full-Text   |   Download PDF [414 KB, uploaded 2 April 2010]   |   Browse Figures
Abstract: This mini-review summarizes the process of reverse-transcription, an obligatory step in retrovirus replication during which the retroviral RNA/DNA-dependent DNA polymerase (RT) copies the single-stranded genomic RNA to generate the double-stranded viral DNA while degrading the genomic RNA via its associated RNase H activity. The hybridization of complementary viral sequences by the nucleocapsid protein (NC) receives a special focus, since it acts to chaperone the strand transfers obligatory for synthesis of the complete viral DNA and flanking long terminal repeats (LTR). Since the physiological microenvironment can impact on reverse-transcription, this mini-review also focuses on factors present in the intra-cellular or extra-cellular milieu that can drastically influence both the timing and the activity of reverse-transcription and hence virus infectivity.
Keywords: reverse transcriptase; nucleocapsid protein; genomic RNA; viral DNA; strand transfers; SEVI reverse transcriptase; nucleocapsid protein; genomic RNA; viral DNA; strand transfers; SEVI
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mougel, M.; Cimarelli, A.; Darlix, J.-L. Implications of the Nucleocapsid and the Microenvironment in Retroviral Reverse Transcription. Viruses 2010, 2, 939-960.

AMA Style

Mougel M, Cimarelli A, Darlix J-L. Implications of the Nucleocapsid and the Microenvironment in Retroviral Reverse Transcription. Viruses. 2010; 2(4):939-960.

Chicago/Turabian Style

Mougel, Marylène; Cimarelli, Andrea; Darlix, Jean-Luc. 2010. "Implications of the Nucleocapsid and the Microenvironment in Retroviral Reverse Transcription." Viruses 2, no. 4: 939-960.


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