Viruses 2010, 2(4), 961-971; doi:10.3390/v2040961
Article

Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name

Jochen Klumpp 1,* email, Richard Calendar 2 email and Martin J. Loessner 1 email
1 Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, Switzerland 2 University of California, 510 Barker Hall, Berkeley, CA 94720, USA
* Author to whom correspondence should be addressed.
Received: 23 February 2010; in revised form: 29 March 2010 / Accepted: 30 March 2010 / Published: 6 April 2010
PDF Full-text Download PDF Full-Text [428 KB, uploaded 6 April 2010 16:16 CEST]
Abstract: Three different Bacillus bacteriophages designated TP21 are known from the literature. We have determined the sequence and structure of the TP21-L genome, and compared it to the other phages. The genome is 37.5 kb in size, possesses fixed invariable genome ends and features the typical modular organization of a temperate siphovirus. TP21-L is neither identical to TP21 isolated by Thorne (TP21-T), as shown by a PCR-based approach nor to TP21 isolated by He et al. (TP21-H), as estimated from phage dimensions. For reasons of clarity, we suggest renaming the different TP21 isolates.
Keywords: TP21; Bacillus; bacteriophage

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Klumpp, J.; Calendar, R.; Loessner, M.J. Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name. Viruses 2010, 2, 961-971.

AMA Style

Klumpp J, Calendar R, Loessner MJ. Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name. Viruses. 2010; 2(4):961-971.

Chicago/Turabian Style

Klumpp, Jochen; Calendar, Richard; Loessner, Martin J. 2010. "Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name." Viruses 2, no. 4: 961-971.

Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert