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Viruses 2010, 2(4), 961-971; doi:10.3390/v2040961
Article

Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name

1,* , 2
 and
1
1 Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, Switzerland 2 University of California, 510 Barker Hall, Berkeley, CA 94720, USA
* Author to whom correspondence should be addressed.
Received: 23 February 2010 / Revised: 29 March 2010 / Accepted: 30 March 2010 / Published: 6 April 2010

Abstract

Three different Bacillus bacteriophages designated TP21 are known from the literature. We have determined the sequence and structure of the TP21-L genome, and compared it to the other phages. The genome is 37.5 kb in size, possesses fixed invariable genome ends and features the typical modular organization of a temperate siphovirus. TP21-L is neither identical to TP21 isolated by Thorne (TP21-T), as shown by a PCR-based approach nor to TP21 isolated by He et al. (TP21-H), as estimated from phage dimensions. For reasons of clarity, we suggest renaming the different TP21 isolates.
Keywords: TP21; Bacillus; bacteriophage TP21; Bacillus; bacteriophage
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Klumpp, J.; Calendar, R.; Loessner, M.J. Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name. Viruses 2010, 2, 961-971.

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