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Viruses 2009, 1(3), 1204-1208; doi:10.3390/v1031204
Article

A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain

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Received: 13 October 2009; in revised form: 28 November 2009 / Accepted: 4 December 2009 / Published: 9 December 2009
(This article belongs to the Special Issue Newly Identified Respiratory Viruses)
View Full-Text   |   Download PDF [85 KB, uploaded 9 December 2009]
Abstract: Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples.
Keywords: influenza; H1N1; real-time; PCR; detection influenza; H1N1; real-time; PCR; detection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Rockett, R.J.; Bialasiewicz, S.; Whiley, D.M.; Bletchly, C.; Faux, C.E.; Lambert, S.B.; Nimmo, G.R.; Nissen, M.D.; Sloots, T.P. A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain. Viruses 2009, 1, 1204-1208.

AMA Style

Rockett RJ, Bialasiewicz S, Whiley DM, Bletchly C, Faux CE, Lambert SB, Nimmo GR, Nissen MD, Sloots TP. A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain. Viruses. 2009; 1(3):1204-1208.

Chicago/Turabian Style

Rockett, Rebecca J.; Bialasiewicz, Seweryn; Whiley, David M.; Bletchly, Cheryl; Faux, Cassandra E.; Lambert, Stephen B.; Nimmo, Graeme R.; Nissen, Michael D.; Sloots, Theo P. 2009. "A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain." Viruses 1, no. 3: 1204-1208.


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