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Viruses 2009, 1(3), 1204-1208; doi:10.3390/v1031204

A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain

1,2,* , 1,2
1 Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children’s Medical Research Institute, Children’s Health Service District, Queensland, Australia 2 Clinical Medical Virology Centre, University of Queensland, Queensland, Australia 3 Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women’s Hospital Campus, Queensland, Australia
* Author to whom correspondence should be addressed.
Received: 13 October 2009 / Revised: 28 November 2009 / Accepted: 4 December 2009 / Published: 9 December 2009
(This article belongs to the Special Issue Newly Identified Respiratory Viruses)
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Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples.
Keywords: influenza; H1N1; real-time; PCR; detection influenza; H1N1; real-time; PCR; detection
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Rockett, R.J.; Bialasiewicz, S.; Whiley, D.M.; Bletchly, C.; Faux, C.E.; Lambert, S.B.; Nimmo, G.R.; Nissen, M.D.; Sloots, T.P. A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain. Viruses 2009, 1, 1204-1208.

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