Viruses 2009, 1(3), 1204-1208; doi:10.3390/v1031204
Article

A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain

Rebecca J. Rockett 1,2,* email, Seweryn Bialasiewicz 1,2 email, David M. Whiley 1,2 email, Cheryl Bletchly 3 email, Cassandra E. Faux 1,2 email, Stephen B. Lambert 1,2 email, Graeme R. Nimmo 3 email, Michael D. Nissen 1,2,3 email and Theo P. Sloots 1,2,3 email
1 Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children’s Medical Research Institute, Children’s Health Service District, Queensland, Australia 2 Clinical Medical Virology Centre, University of Queensland, Queensland, Australia 3 Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women’s Hospital Campus, Queensland, Australia
* Author to whom correspondence should be addressed.
Received: 13 October 2009; in revised form: 28 November 2009 / Accepted: 4 December 2009 / Published: 9 December 2009
(This article belongs to the Special Issue Newly Identified Respiratory Viruses)
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Abstract: Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples.
Keywords: influenza; H1N1; real-time; PCR; detection

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Cite This Article

MDPI and ACS Style

Rockett, R.J.; Bialasiewicz, S.; Whiley, D.M.; Bletchly, C.; Faux, C.E.; Lambert, S.B.; Nimmo, G.R.; Nissen, M.D.; Sloots, T.P. A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain. Viruses 2009, 1, 1204-1208.

AMA Style

Rockett RJ, Bialasiewicz S, Whiley DM, Bletchly C, Faux CE, Lambert SB, Nimmo GR, Nissen MD, Sloots TP. A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain. Viruses. 2009; 1(3):1204-1208.

Chicago/Turabian Style

Rockett, Rebecca J.; Bialasiewicz, Seweryn; Whiley, David M.; Bletchly, Cheryl; Faux, Cassandra E.; Lambert, Stephen B.; Nimmo, Graeme R.; Nissen, Michael D.; Sloots, Theo P. 2009. "A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain." Viruses 1, no. 3: 1204-1208.

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