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Mar. Drugs 2011, 9(2), 184-195; doi:10.3390/md9020184
Article

Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica

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Received: 17 December 2010; in revised form: 4 January 2011 / Accepted: 14 January 2011 / Published: 31 January 2011
(This article belongs to the Special Issue Enzymes from the Sea: Sources, Molecular Biology and Bioprocesses)
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Abstract: The diversity and cold-active hydrolytic enzymes of culturable bacteria associated with sandy sediment from Nella Fjord, Eastern Antarctica (69°22′6″ S, 76°21′45″ E) was investigated. A total of 33 aerobic heterotrophic bacterial strains were isolated at 4 °C. These bacterial isolates could be sorted into 18 phylotypes based on the 16S rRNA gene sequence belonging to four phyla, namely Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria. Only seven isolates were psychrophilic, 15 isolates were moderately psychrophilic, and 11 isolates were psychrotolerant. More than 72% of the isolates required sodium chloride to grow. Esterase, b-glucosidase and proteases activities at 4 °C were detected in more than 45% of the strains while approximately 21%, 15% and 12% of the strains possessed lipase, amylase and chitinase, respectively. These results indicate that a relatively high culturable bacterial diversity is present within marine sediment of Nella Fjord and it could serve as an ideal candidate region for bioprospecting.
Keywords: psychrophilic; heterotrophic bacteria; diversity; cold-active enzymes; Antarctica   psychrophilic; heterotrophic bacteria; diversity; cold-active enzymes; Antarctica  
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yu, Y.; Li, H.-R.; Zeng, Y.-X.; Chen, B. Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica. Mar. Drugs 2011, 9, 184-195.

AMA Style

Yu Y, Li H-R, Zeng Y-X, Chen B. Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica. Marine Drugs. 2011; 9(2):184-195.

Chicago/Turabian Style

Yu, Yong; Li, Hui-Rong; Zeng, Yin-Xin; Chen, Bo. 2011. "Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica." Mar. Drugs 9, no. 2: 184-195.



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