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Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China
Biomedical Analysis Center, Tsinghua University, Beijing 100084, China
* Author to whom correspondence should be addressed.
Received: 2 January 2011; in revised form: 15 January 2011 / Accepted: 19 January 2011 / Published: 21 January 2011
Abstract: An alginate lyase-producing bacterial strain, Pseudoalteromonas sp. SM0524, was screened from marine rotten kelp. In an optimized condition, the production of alginate lyase from Pseudoalteromonas sp. SM0524 reached 62.6 U/mL, suggesting that strain SM0524 is a good producer of alginate lyases. The bifunctional alginate lyasealy-SJ02 secreted by strain SM0524 was purified. Aly-SJ02 had an apparent molecular mass of 32 kDa. The optimal temperature and pH of aly-SJ02 toward sodium alginate was 50 °C and 8.5, respectively. The half life period of aly-SJ02 was 41 min at 40 °C and 20 min at 50 °C. Aly-SJ02 was most stable at pH 8.0. N-terminal sequence analysis suggested that aly-SJ02 may be an alginate lyase of polysaccharide lyase family 18. Aly-SJ02 showed activities toward both polyG (α-L-guluronic acid) and polyM (β-D-mannuronic acid), indicating that it is a bifunctional alginate lyase. Aly-SJ02 had lower Km toward polyG than toward polyM and sodium alginate. Thin layer chromatography and ESI-MS analyses showed that aly-SJ02 mainly released dimers and trimers from polyM and alginate, and trimers and tetramers from polyG, which suggests that aly-SJ02 may be a good tool to produce dimers and trimers from alginate.
Keywords: bifunctional alginate lyase; Pseudoalteromonas sp. SM0524; alginate
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MDPI and ACS Style
Li, J.-W.; Dong, S.; Song, J.; Li, C.-B.; Chen, X.-L.; Xie, B.-B.; Zhang, Y.-Z. Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524. Mar. Drugs 2011, 9, 109-123.
Li J-W, Dong S, Song J, Li C-B, Chen X-L, Xie B-B, Zhang Y-Z. Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524. Marine Drugs. 2011; 9(1):109-123.
Li, Jian-Wei; Dong, Sheng; Song, Jie; Li, Chun-Bo; Chen, Xiu-Lan; Xie, Bin-Bin; Zhang, Yu-Zhong. 2011. "Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524." Mar. Drugs 9, no. 1: 109-123.