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Marine Bacterial Sialyltransferases
Mar. Drugs 2011, 9(1), 109-123; doi:10.3390/md9010109

Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524

1,* , 1
1 State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China 2 Biomedical Analysis Center, Tsinghua University, Beijing 100084, China
* Author to whom correspondence should be addressed.
Received: 2 January 2011 / Revised: 15 January 2011 / Accepted: 19 January 2011 / Published: 21 January 2011
(This article belongs to the Special Issue Enzymes from the Sea: Sources, Molecular Biology and Bioprocesses)
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An alginate lyase-producing bacterial strain, Pseudoalteromonas sp. SM0524, was screened from marine rotten kelp. In an optimized condition, the production of alginate lyase from Pseudoalteromonas sp. SM0524 reached 62.6 U/mL, suggesting that strain SM0524 is a good producer of alginate lyases. The bifunctional alginate lyasealy-SJ02 secreted by strain SM0524 was purified. Aly-SJ02 had an apparent molecular mass of 32 kDa. The optimal temperature and pH of aly-SJ02 toward sodium alginate was 50 °C and 8.5, respectively. The half life period of aly-SJ02 was 41 min at 40 °C and 20 min at 50 °C. Aly-SJ02 was most stable at pH 8.0. N-terminal sequence analysis suggested that aly-SJ02 may be an alginate lyase of polysaccharide lyase family 18. Aly-SJ02 showed activities toward both polyG (α-L-guluronic acid) and polyM (β-D-mannuronic acid), indicating that it is a bifunctional alginate lyase. Aly-SJ02 had lower Km toward polyG than toward polyM and sodium alginate. Thin layer chromatography and ESI-MS analyses showed that aly-SJ02 mainly released dimers and trimers from polyM and alginate, and trimers and tetramers from polyG, which suggests that aly-SJ02 may be a good tool to produce dimers and trimers from alginate.
Keywords: bifunctional alginate lyase; Pseudoalteromonas sp. SM0524; alginate bifunctional alginate lyase; Pseudoalteromonas sp. SM0524; alginate
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Li, J.-W.; Dong, S.; Song, J.; Li, C.-B.; Chen, X.-L.; Xie, B.-B.; Zhang, Y.-Z. Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524. Mar. Drugs 2011, 9, 109-123.

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