Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like Approach

doi:10.3390/s90301599

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Sensors 2009, 9(3), 1599-1608; doi:10.3390/s90301599

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Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like Approach

Diego J. Kormes¹ and Eduardo Cortón^2,*

¹ Biosensors and Bioanalysis Group, FCEN, UBA / Department of Biochemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, (1428) Buenos Aires, Argentina ² Biosensors and Bioanalysis Group, FCEN, UBA and CONICET / Department of Biochemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, (1428) Buenos Aires, Argentina

* Author to whom correspondence should be addressed.

Received: 12 December 2008; in revised form: 21 January 2009 / Accepted: 6 March 2009 / Published: 9 March 2009

(This article belongs to the Section Biosensors)

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Abstract: Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol^-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed.

Keywords: Biosensors; microbial metabolism; Arrhenius plot; activation energy; Saccharomyces cerevisiae; glucose transport

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MDPI and ACS Style

Kormes, D.J.; Cortón, E. Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like Approach. Sensors 2009, 9, 1599-1608.

AMA Style

Kormes D.J., Cortón E. Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like Approach. Sensors. 2009; 9(3):1599-1608.

Chicago/Turabian Style

Kormes, Diego J.; Cortón, Eduardo. 2009. "Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like Approach." Sensors 9, no. 3: 1599-1608.

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