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DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool
Department of Chemistry, Pohang University of Science & Technology, Pohang 790-784, Korea
Interdisciplinary School of Green Energy Engineering, Ulsan National Institute of Science & Technology, Ulsan 689-805, Korea
* Author to whom correspondence should be addressed.
Received: 30 September 2009; in revised form: 28 October 2009 / Accepted: 11 November 2009 / Published: 26 November 2009
Abstract: Recent advances in label free DNA hybridization sensors employing electrochemical impedance spectroscopy (EIS) as a detection tool are reviewed. These sensors are based on the modulation of the blocking ability of an electrode modified with a probe DNA by an analyte, i.e., target DNA. The probe DNA is immobilized on a self-assembled monolayer, a conducting polymer film, or a layer of nanostructures on the electrode such that desired probe DNA would selectively hybridize with target DNA. The rate of charge transfer from the electrode thus modified to a redox indicator, e.g., [Fe(CN)6]3–/4–, which is measured by EIS in the form of charge transfer resistance (Rct), is modulated by whether or not, as well as how much, the intended target DNA is selectively hybridized. Efforts made to enhance the selectivity as well as the sensitivity of DNA sensors and to reduce the EIS measurement time are briefly described along with brief future perspectives in developing DNA sensors.
Keywords: DNA; self-assembled monolayers; conducting polymers; nanostructures; EIS
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MDPI and ACS Style
Park, J.-Y.; Park, S.-M. DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool. Sensors 2009, 9, 9513-9532.
Park J-Y, Park S-M. DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool. Sensors. 2009; 9(12):9513-9532.
Park, Jin-Young; Park, Su-Moon. 2009. "DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool." Sensors 9, no. 12: 9513-9532.