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Sensors 2009, 9(12), 10356-10388; doi:10.3390/s91210356

Aptamer-Functionalized Nano-Biosensors

1,*  and 2,*
1 Department of Applied Science, National Taitung University, 684, Section 1, Chunghua Road, Taitung, 95002, Taiwan 2 Institute of Bioscience and Biotechnology, National Taiwan Ocean University, 2 Beining Road, Keelung, 20224, Taiwan
* Authors to whom correspondence should be addressed.
Received: 22 October 2009 / Revised: 3 December 2009 / Accepted: 3 December 2009 / Published: 21 December 2009
(This article belongs to the Special Issue DNA Sensors and Biosensors)
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Nanomaterials have become one of the most interesting sensing materials because of their unique size- and shape-dependent optical properties, high surface energy and surface-to-volume ratio, and tunable surface properties. Aptamers are oligonucleotides that can bind their target ligands with high affinity. The use of nanomaterials that are bioconjugated with aptamers for selective and sensitive detection of analytes such as small molecules, metal ions, proteins, and cells has been demonstrated. This review focuses on recent progress in the development of biosensors by integrating functional aptamers with different types of nanomaterials, including quantum dots, magnetic nanoparticles (NPs), metallic NPs, and carbon nanotubes. Colorimetry, fluorescence, electrochemistry, surface plasmon resonance, surface-enhanced Raman scattering, and magnetic resonance imaging are common detection modes for a broad range of analytes with high sensitivity and selectivity when using aptamer bioconjugated nanomaterials (Apt-NMs). We highlight the important roles that the size and concentration of nanomaterials, the secondary structure and density of aptamers, and the multivalent interactions play in determining the specificity and sensitivity of the nanosensors towards analytes. Advantages and disadvantages of the Apt-NMs for bioapplications are focused.
Keywords: nanomaterials; aptamers; biosensors; metal ions; proteins; cells nanomaterials; aptamers; biosensors; metal ions; proteins; cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chiu, T.-C.; Huang, C.-C. Aptamer-Functionalized Nano-Biosensors. Sensors 2009, 9, 10356-10388.

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