• Submit to Sensors Login Register
• MDPI
• Journals A-Z
• For Authors
• For Editors
• About
• Open Access Policy

• Abstract
• Full-Text PDF [464 KB]
• Supplement File 1

• Article Statistics
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Prieto-Simón, B
• Campàs, M
• Andreescu, S
• Marty, J
• [+] on Google Scholar
• Prieto-Simón, B
• Campàs, M
• Andreescu, S
• Marty, J
• [+] on PubMed
• Prieto-Simón, B
• Campàs, M
• Andreescu, S
• Marty, J

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Sensors 2006, 6(10), 1161-1186; doi:10.3390/s6101161

Review

Trends in Flow-based Biosensing Systems for Pesticide Assessment

Beatriz Prieto-Simón ¹ , Mònica Campàs ¹ , Silvana Andreescu ²  and Jean-Louis Marty ^1,*

¹ BIOMEM group, Université de Perpignan, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France ² Department of Chemistry and Biomolecular Sciences, Clarkson University, Potsdam, NY 13699-5810, USA

* Author to whom correspondence should be addressed.

Received: 29 July 2006 / Accepted: 29 September 2006 / Published: 1 October 2006

(This article belongs to the Special Issue Sensors in Flow Analysis)

Download PDF Full-Text [464 KB, uploaded 20 June 2008 16:51 CEST]

Abstract: This review gives a survey on the state of the art of pesticide detection usingflow-based biosensing systems for sample screening. Although immunosensor systems havebeen proposed as powerful pesticide monitoring tools, this review is mainly focused onenzyme-based biosensors, as they are the most commonly employed when using a flowsystem. Among the different detection methods able to be integrated into flow-injectionanalysis (FIA) systems, the electrochemical ones will be treated in more detail, due to theirhigh sensitivity, simple sample pretreatment, easy operational procedures and real-timedetection. During the last decade, new trends have been emerging in order to increase theenzyme stability, the sensitivity and selectivity of the measurements, and to lower thedetection limits. These approaches are based on (i) the design of novel matrices for enzymeimmobilisation, (ii) new manifold configurations of the FIA system, sometimes includingminiaturisation or lab-on-chip protocols thanks to micromachining technology, (iii) the useof cholinesterase enzymes either from various commercial sources or genetically modifiedwith the aim of being more sensitive, (iv) the incorporation of other highly specificenzymes, such as organophosphate hydrolase (OPH) or parathion hydrolase (PH) and (v) thecombination of different electrochemical methods of detection. This article discusses thesenovel strategies and their advantages and limitations.

Keywords: enzyme inhibition; acetylcholinesterase (AChE); pesticide; flow-injection analysis (FIA) System

Supplementary Files

• Supplementary File 1:

  DOC-Document (DOC, 113 KB)

Article Statistics

Cite This Article