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Sensors 2015, 15(6), 13839-13850; doi:10.3390/s150613839

A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe

1
School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China
2
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 29 April 2015 / Revised: 29 May 2015 / Accepted: 9 June 2015 / Published: 11 June 2015
(This article belongs to the Special Issue On-Chip Sensors)
View Full-Text   |   Download PDF [546 KB, uploaded 12 June 2015]   |  

Abstract

A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or “artificial antibody”, was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the “aptamer beacon”, highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics. View Full-Text
Keywords: Love wave; biosensing; PSA; aptamer; stem-loop probe; microfluidic; PDMS Love wave; biosensing; PSA; aptamer; stem-loop probe; microfluidic; PDMS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhang, F.; Li, S.; Cao, K.; Wang, P.; Su, Y.; Zhu, X.; Wan, Y. A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe. Sensors 2015, 15, 13839-13850.

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