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Open AccessCommunication
Sensors 2015, 15(4), 8605-8614; doi:10.3390/s150408605

Rapid Detection of Ebola Virus with a Reagent-Free, Point-of-Care Biosensor

1
Department of Emergency Medicine, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
2
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
3
Sandia National Laboratories, Albuquerque, NM 87185, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Stephane Evoy
Received: 27 February 2015 / Revised: 30 March 2015 / Accepted: 9 April 2015 / Published: 14 April 2015
(This article belongs to the Special Issue Biosensors for Pathogen Detection)
View Full-Text   |   Download PDF [2081 KB, uploaded 14 April 2015]   |  

Abstract

Surface acoustic wave (SAW) sensors can rapidly detect Ebola antigens at the point-of-care without the need for added reagents, sample processing, or specialized personnel. This preliminary study demonstrates SAW biosensor detection of the Ebola virus in a concentration-dependent manner. The detection limit with this methodology is below the average level of viremia detected on the first day of symptoms by PCR. We observe a log-linear sensor response for highly fragmented Ebola viral particles, with a detection limit corresponding to 1.9 × 104 PFU/mL prior to virus inactivation. We predict greatly improved sensitivity for intact, infectious Ebola virus. This point-of-care methodology has the potential to detect Ebola viremia prior to symptom onset, greatly enabling infection control and rapid treatment. This biosensor platform is powered by disposable AA batteries and can be rapidly adapted to detect other emerging diseases in austere conditions. View Full-Text
Keywords: pathogens; infectious diseases; viruses; piezoelectric; biosensors; diagnosis; SAW; surface acoustic wave pathogens; infectious diseases; viruses; piezoelectric; biosensors; diagnosis; SAW; surface acoustic wave
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Baca, J.T.; Severns, V.; Lovato, D.; Branch, D.W.; Larson, R.S. Rapid Detection of Ebola Virus with a Reagent-Free, Point-of-Care Biosensor. Sensors 2015, 15, 8605-8614.

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