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Sensors 2015, 15(3), 5376-5389; doi:10.3390/s150305376

A Portable Automatic Endpoint Detection System for Amplicons of Loop Mediated Isothermal Amplification on Microfluidic Compact Disk Platform

1
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
2
Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
3
Microbiology Unit, Institute of Biological Sciences, Faculty of Science, University of Malaya 50603 Kuala Lumpur, Malaysia
4
Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia
5
Department of Biomedical Engineering, Inje University, Gimhae 621-749, South Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Stephane Evoy
Received: 16 December 2014 / Revised: 19 January 2015 / Accepted: 28 January 2015 / Published: 5 March 2015
(This article belongs to the Special Issue Biosensors for Pathogen Detection)
View Full-Text   |   Download PDF [2067 KB, uploaded 5 March 2015]   |  

Abstract

In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10−3 ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment. View Full-Text
Keywords: pathogen; Salmonella; food safety; diagnosis; LAMP; multiplexed detection; endpoint detection system; microfluidics; compact disc; lab-on-a-CD pathogen; Salmonella; food safety; diagnosis; LAMP; multiplexed detection; endpoint detection system; microfluidics; compact disc; lab-on-a-CD
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Uddin, S.M.; Ibrahim, F.; Sayad, A.A.; Thiha, A.; Pei, K.X.; Mohktar, M.S.; Hashim, U.; Cho, J.; Thong, K.L. A Portable Automatic Endpoint Detection System for Amplicons of Loop Mediated Isothermal Amplification on Microfluidic Compact Disk Platform. Sensors 2015, 15, 5376-5389.

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