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Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma
Department of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA
School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY 14642, USA
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 16 July 2013; in revised form: 6 September 2013 / Accepted: 11 September 2013 / Published: 17 September 2013
Abstract: The isolation of hematopoietic stem and progenitor cells (HSPCs) is critical for transplantation therapy and HSPC research, however current isolation techniques can be prohibitively expensive, time-consuming, and produce variable results. Selectin-coated microtubes have shown promise in rapidly isolating HSPCs from human bone marrow, but further purification of HSPCs remains a challenge. Herein, a biomimetic device for HSPC isolation is presented to mimic the acidic vascular microenvironment during trauma, which can enhance the binding frequency between L-selectin and its counter-receptor PSGL-1 and HSPCs. Under acidic pH conditions, L-selectin coated microtubes enhanced CD34+ HSPC adhesion, as evidenced by decreased cell rolling velocity and increased rolling flux. Dynamic light scattering was utilized as a novel sensor to confirm an L-selectin conformational change under acidic conditions, as previously predicted by molecular dynamics. These results suggest that mimicking the acidic conditions of trauma can induce a conformational extension of L-selectin, which can be utilized for flow-based, clinical isolation of HSPCs.
Keywords: CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH
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MDPI and ACS Style
Cao, T.M.; Mitchell, M.J.; Liesveld, J.; King, M.R. Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma. Sensors 2013, 13, 12516-12526.
Cao TM, Mitchell MJ, Liesveld J, King MR. Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma. Sensors. 2013; 13(9):12516-12526.
Cao, Thong M.; Mitchell, Michael J.; Liesveld, Jane; King, Michael R. 2013. "Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma." Sensors 13, no. 9: 12516-12526.