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Sensors 2013, 13(9), 12516-12526; doi:10.3390/s130912516

Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma

1 Department of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA 2 School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY 14642, USA These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 16 July 2013 / Revised: 6 September 2013 / Accepted: 11 September 2013 / Published: 17 September 2013
(This article belongs to the Special Issue Biomimetic Receptors and Sensors)
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The isolation of hematopoietic stem and progenitor cells (HSPCs) is critical for transplantation therapy and HSPC research, however current isolation techniques can be prohibitively expensive, time-consuming, and produce variable results. Selectin-coated microtubes have shown promise in rapidly isolating HSPCs from human bone marrow, but further purification of HSPCs remains a challenge. Herein, a biomimetic device for HSPC isolation is presented to mimic the acidic vascular microenvironment during trauma, which can enhance the binding frequency between L-selectin and its counter-receptor PSGL-1 and HSPCs. Under acidic pH conditions, L-selectin coated microtubes enhanced CD34+ HSPC adhesion, as evidenced by decreased cell rolling velocity and increased rolling flux. Dynamic light scattering was utilized as a novel sensor to confirm an L-selectin conformational change under acidic conditions, as previously predicted by molecular dynamics. These results suggest that mimicking the acidic conditions of trauma can induce a conformational extension of L-selectin, which can be utilized for flow-based, clinical isolation of HSPCs.
Keywords: CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Cao, T.M.; Mitchell, M.J.; Liesveld, J.; King, M.R. Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma. Sensors 2013, 13, 12516-12526.

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