Next Article in Journal
Quantitative Assessment of Birefringent Skin Structures in Scattered Light Confocal Imaging Using Radially Polarized Light
Next Article in Special Issue
Highly Selective Fluorescent Sensing of Proteins Based on a Fluorescent Molecularly Imprinted Nanosensor
Previous Article in Journal
Data Processing and Quality Evaluation of a Boat-Based Mobile Laser Scanning System
Previous Article in Special Issue
Optimizing the Thermal Read-Out Technique for MIP-Based Biomimetic Sensors: Towards Nanomolar Detection Limits
Sensors 2013, 13(9), 12516-12526; doi:10.3390/s130912516

Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma

1 Department of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA 2 School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY 14642, USA These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 16 July 2013 / Revised: 6 September 2013 / Accepted: 11 September 2013 / Published: 17 September 2013
(This article belongs to the Special Issue Biomimetic Receptors and Sensors)
View Full-Text   |   Download PDF [543 KB, 21 June 2014; original version 21 June 2014]   |   Browse Figures


The isolation of hematopoietic stem and progenitor cells (HSPCs) is critical for transplantation therapy and HSPC research, however current isolation techniques can be prohibitively expensive, time-consuming, and produce variable results. Selectin-coated microtubes have shown promise in rapidly isolating HSPCs from human bone marrow, but further purification of HSPCs remains a challenge. Herein, a biomimetic device for HSPC isolation is presented to mimic the acidic vascular microenvironment during trauma, which can enhance the binding frequency between L-selectin and its counter-receptor PSGL-1 and HSPCs. Under acidic pH conditions, L-selectin coated microtubes enhanced CD34+ HSPC adhesion, as evidenced by decreased cell rolling velocity and increased rolling flux. Dynamic light scattering was utilized as a novel sensor to confirm an L-selectin conformational change under acidic conditions, as previously predicted by molecular dynamics. These results suggest that mimicking the acidic conditions of trauma can induce a conformational extension of L-selectin, which can be utilized for flow-based, clinical isolation of HSPCs.
Keywords: CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Cao, T.M.; Mitchell, M.J.; Liesveld, J.; King, M.R. Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma. Sensors 2013, 13, 12516-12526.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert