Next Article in Journal
Assessing Routing Strategies for Cognitive Radio Sensor Networks
Next Article in Special Issue
Development of a Sweetness Sensor for Aspartame, a Positively Charged High-Potency Sweetener
Previous Article in Journal
A Low Density Microarray Method for the Identification of Human Papillomavirus Type 18 Variants
Previous Article in Special Issue
Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma
Sensors 2013, 13(10), 12994-13004; doi:10.3390/s131012994

Highly Selective Fluorescent Sensing of Proteins Based on a Fluorescent Molecularly Imprinted Nanosensor

Received: 24 July 2013 / Revised: 5 August 2013 / Accepted: 16 August 2013 / Published: 26 September 2013
(This article belongs to the Special Issue Biomimetic Receptors and Sensors)
View Full-Text   |   Download PDF [1509 KB, uploaded 21 June 2014]   |   Browse Figures


A fluorescent molecularly imprinted nanosensor was obtained by grafting imprinted polymer onto the surface of multi-wall carbon nanotubes and post-imprinting treatment with fluorescein isothiocyanate (FITC). The fluorescence of lysozyme-imprinted polymer (Lys-MIP) was quenched more strongly by Lys than that of nonimprinted polymer (NIP), which indicated that the Lys-MIP could recognize Lys. The resulted imprinted material has the ability to selectively sense a target protein, and an imprinting factor of 3.34 was achieved. The Lys-MIP also showed selective detection for Lys among other proteins such as cytochrome C (Cyt C), hemoglobin (HB) and bovine serum albumin (BSA) due to the imprinted sites in the Lys-MIP. This approach combines the high selectivity of surface molecular imprinting technology and fluorescence, and converts binding events into detectable signals by monitoring fluorescence spectra. Therefore, it will have further applications for Lys sensing.
Keywords: molecularly imprinted polymers; protein; fluorescent sensing molecularly imprinted polymers; protein; fluorescent sensing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Deng, Q.; Wu, J.; Zhai, X.; Fang, G.; Wang, S. Highly Selective Fluorescent Sensing of Proteins Based on a Fluorescent Molecularly Imprinted Nanosensor. Sensors 2013, 13, 12994-13004.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert