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Highly Selective Fluorescent Sensing of Proteins Based on a Fluorescent Molecularly Imprinted Nanosensor
Department of Science, Tianjin University of Science and Technology, Tianjin 300457, China
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 24 July 2013; in revised form: 5 August 2013 / Accepted: 16 August 2013 / Published: 26 September 2013
Abstract: A fluorescent molecularly imprinted nanosensor was obtained by grafting imprinted polymer onto the surface of multi-wall carbon nanotubes and post-imprinting treatment with fluorescein isothiocyanate (FITC). The fluorescence of lysozyme-imprinted polymer (Lys-MIP) was quenched more strongly by Lys than that of nonimprinted polymer (NIP), which indicated that the Lys-MIP could recognize Lys. The resulted imprinted material has the ability to selectively sense a target protein, and an imprinting factor of 3.34 was achieved. The Lys-MIP also showed selective detection for Lys among other proteins such as cytochrome C (Cyt C), hemoglobin (HB) and bovine serum albumin (BSA) due to the imprinted sites in the Lys-MIP. This approach combines the high selectivity of surface molecular imprinting technology and fluorescence, and converts binding events into detectable signals by monitoring fluorescence spectra. Therefore, it will have further applications for Lys sensing.
Keywords: molecularly imprinted polymers; protein; fluorescent sensing
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MDPI and ACS Style
Deng, Q.; Wu, J.; Zhai, X.; Fang, G.; Wang, S. Highly Selective Fluorescent Sensing of Proteins Based on a Fluorescent Molecularly Imprinted Nanosensor. Sensors 2013, 13, 12994-13004.
Deng Q, Wu J, Zhai X, Fang G, Wang S. Highly Selective Fluorescent Sensing of Proteins Based on a Fluorescent Molecularly Imprinted Nanosensor. Sensors. 2013; 13(10):12994-13004.
Deng, Qiliang; Wu, Jianhua; Zhai, Xiaorui; Fang, Guozhen; Wang, Shuo. 2013. "Highly Selective Fluorescent Sensing of Proteins Based on a Fluorescent Molecularly Imprinted Nanosensor." Sensors 13, no. 10: 12994-13004.