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Sensors 2013, 13(7), 8551-8563; doi:10.3390/s130708551
Article

Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode

1
,
1,* , 2
,
1
,
3
 and
4
1 College of Engineering, Shanxi Agriculture University, Taigu 030801, China 2 College of Electrical Engineering, Hebei United University, Tangshan 063009, China 3 College of Animal Science and Technology, Shanxi Agriculture University, Taigu 030801, China 4 College of Food Science & Technology, Shanxi Agriculture University, Taigu 030801, China
* Author to whom correspondence should be addressed.
Received: 20 May 2013 / Revised: 21 June 2013 / Accepted: 21 June 2013 / Published: 4 July 2013
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Abstract

A rapid and simple method for quantitative monitoring of Brucella melitensis using electrochemical impedance spectroscopy (EIS) is reported for the first time. The label-free immunosensors were fabricated by immobilizing Brucella melitensis antibody on the surface of gold nanoparticle-modified screen-printed carbon electrodes (GNP-SPCEs). Cyclic voltammetry (CV) and EIS were used to characterize the Brucella melitensis antigen interaction on the surface of GNP-SPCEs with antibody. A general electronic equivalent model of an electrochemical cell was introduced for interpretation of the impedance components of the system. The results showed that the change in electron-transfer resistance (Rct) was significantly different due to the binding of Brucella melitensis cells. A linear relationship between the Rct variation and logarithmic value of the cell concentration was found from 4 × 104 to 4 × 106 CFU/mL in pure culture. The label-free impedance biosensor was able to detect as low as 1 × 104 and 4 × 105 CFU/mL of Brucella melitensis in pure culture and milk samples, respectively, in less than 1.5 h. Moreover, a good selectivity versus Escherichia coli O157:H7 and Staphylococcus aureus cells was obtained for our developed immunosensor demonstrating its specificity towards only Brucella melitensis.
Keywords: quantitative detection; Brucella melitensis; label-free immunosensor; screen-printed carbon electrode; gold nanoparticle quantitative detection; Brucella melitensis; label-free immunosensor; screen-printed carbon electrode; gold nanoparticle
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Wu, H.; Zuo, Y.; Cui, C.; Yang, W.; Ma, H.; Wang, X. Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode. Sensors 2013, 13, 8551-8563.

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